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Browsing Modes for Business Users
-
Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
-
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and will not have access to any other carts
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Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Anti-Burosumab Antibodies
Antibodies for bioanalysis and drug monitoring of burosumab
Develop highly selective and sensitive PK and ADA assays for the humanized antibody drug burosumab (Crysvita®) using our range of ready-made anti-idiotypic antibodies.
- Highly specific to burosumab and the burosumab/human FGF-23-His complex
- Fully human surrogate positive control or calibrator
- Sequence defined, well characterized reagents with secure supply
- Recombinant production, stringent quality control for batch-to-batch consistency
- Non-animal-derived antibodies, reducing the use of animals in science
Table 1. Antibodies specific to burosumab.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
|---|---|---|---|---|---|---|
|
Burosumab Inhibitory Type 1 |
HCA459 |
AbD63865ia |
hIgG1 |
0.1 |
ADA assay |
|
|
HCA460 |
AbD63870ia |
hIgG1 |
0.6 |
ADA assay |
||
|
HCA461 |
AbD63872ia |
hIgG1 |
6.2 |
ADA assay |
||
|
TZA0109 |
AbD63869ad |
Fab-F-Spy2-H1 |
0.4 |
PK bridging ELISA (capture) |
||
|
TZA0110 |
AbD63865ad |
Fab-F-Spy2-H1 |
0.1 |
PK bridging ELISA (detection) |
||
|
TZA0110P |
AbD63865pap |
Fab2-FH-X22-HRP2 |
0.1 |
PK bridging ELISA (detection) |
||
|
Burosumab/hFGF-23-His Complex Specific Type 3 |
TZA0111 |
AbD64797ad |
Fab-F-Spy2-H1 |
1 |
PK ELISA antigen capture format |
|
|
TZA0111P |
AbD64797pap |
Fab2-FH-X22-HRP2 |
1 |
PK ELISA antigen capture format |
* Affinity measured in the monovalent Fab format.
1 Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
2 Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.
Anti-Burosumab Inhibitory Antibodies (Type 1)
Type 1 anti-burosumab antibodies inhibit the binding of the drug burosumab to its target, human fibroblast growth factor 23 (FGF-23). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format, they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.
Anti-Burosumab/hFGF-23-His Complex Specific Antibodies (Type 3)
The Type 3 antibodies specifically recognize the drug-target complex, detecting burosumab only when it is bound to hFGF-23-His. They are suitable as a detection antibody in a PK antigen capture assay.
ELISA Protocols to Get You Started
PK Bridging ELISA
Fig. 1. Burosumab PK bridging ELISA using antibodies TZA0109 (capture) and TZA0110P (detection).
Schematic image of PK bridging ELISA detecting free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Fab format (purple), labeled with HRP.
PK Antigen Capture ELISA
Fig. 2. Burosumab PK antigen capture ELISA using hFGF-23-His and TZA0111P.
Schematic image of PK antigen capture ELISA.??? Drug target (red), monoclonal antibody drug (gold), drug-target complex detection antibody, monovalent Fab format (purple), labeled with HRP.
ADA Bridging ELISA
Fig. 3. Burosumab ADA bridging ELISA using HCA459, HCA460 and HCA461.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
Resources
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.