Block ACE

//www.bio-rad-antibodies.com/block-ace-accessory-reagent-buf029.html?RenderPageSpecific=1
Block ACE gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • Block ACE thumbnail image 1
  • Block ACE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF029E, WBdatasheet pdfdatasheet pdf20 X 4g
    BUF029
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western blotting. It may also be used for dilution of antibodies and for washing procedures in the above assays.

      Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves.

      For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution.

      For blocking in Western Blotting applications, we recommend using the reconstituted solution neat.

      For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v.

      For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution.
    • Intended Use
    • Product Form
    • Reconstitution
      Reconstitute each 4g sachet in 100ml distilled water.
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Prior to reconstitution, 2 years from date of despatch. After reconstitution, 1 week at 4oC.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/4
      Western BlottingNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate negative/positive controls
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Block ACE Formats

    Formats Applications Sizes available
    Block ACE : Reagent E, WB 20 X 4g
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

              Write your review

              You may also be interested in...