Trastuzumab Antibody | AbD16712_hIgG1

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Trastuzumab Antibody | AbD16712_hIgG1 gallery image 1

Human anti Trastuzumab (Herceptin®) specificity ELISA

A microtiter plate was coated with various antigens (A: 20% human serum, B: IgG1/kappa from human myeloma plasma, C: Rituximab, D: Bevacizumab, E: Ustekinumab, F: Infliximab, G: Adalimumab, H: Trastuzumab) as coating components and HRP conjugated Human anti Trastuzumab (HCA176) was used as the detection reagent

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Human anti Trastuzumab (Herceptin®) bridging ELISA

Trastuzumab was used as the coating reagent; followed by Human anti Trastuzumab (HCA176) in 10% human serum and HRP conjugated Trastuzumab as the detection reagent

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Human anti Trastuzumab (Herceptin®) bridging ELISA

Human anti Trastuzumab, HCA166 (black) or HCA169 (red) was used as the coating reagent; Trastuzumab as the antigen and HRP conjugated Human anti Trastuzumab (HCA176) as the detection reagent

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  • Human anti Trastuzumab:HRP
  • Human anti Trastuzumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-trastuzumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical assay development. It can be used as a reference standard in an ADA bridging ELISA and as detection antibody in a PK bridging ELISA with HCA169.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD16712_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA176PEdatasheet pdfdatasheet pdf0.1 mg
      HCA176P
      HCA176Edatasheet pdfdatasheet pdf0.1 mg
      HCA176
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti Trastuzumab, clone AbD16712_hIgG1, is an anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody drug trastuzumab, also known as Herceptin®. Clone AbD16712_hIgG can be used to measure the levels of trastuzumab and biosimilar products using bioanalytical assays.

        In the HRP-conjugated format clone AbD16712_hIgG1 is ideal for use as a detection antibody in pharmacokinetic (PK) assays. It is a fully human antibody and therefore may also be used as a calibrator in immune response assays to measure the anti-drug-antibody (ADA) response in patient sera.

        Trastuzumab is a drug used in the treatment of HER2 postive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells

        View a summary of all anti-trastuzumab antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Trastuzumab (Herceptin®)
      • Purity
      • Approx. Protein Concentrations
        Antibody concentration 0.1 mg/ml
        Antibody concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline.
      • Storage
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin® is a registered trademark of Genentech, Inc.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin is a registered trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used as a detection reagent in a sandwich ELISA together with HCA169 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Trastuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Trastuzumab Antibody : Purified AbD16712_hIgG1 E 0.1 mg
      Trastuzumab Antibody : HRP AbD16712_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human IgG1 LambdaHCA0490.1 mgE
          HCA049

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            MCA647P
            LYNX Rapid HRP Antibody Conjugation KitLNK005P1 Conjugation For 20mg AntibodyCJ
            LNK005P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            LYNX Rapid HRP Antibody Conjugation KitLNK003P1 Conjugation For 4mg AntibodyCJ
            LNK003P
            LYNX Rapid HRP Antibody Conjugation KitLNK006P3 Conjugations For 40µg AntibodyCJ
            LNK006P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            LYNX Rapid HRP Antibody Conjugation KitLNK004P5 Conjugations For 4mg AntibodyCJ
            LNK004P
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1670.1 mgE
            HCA167
            Human anti TrastuzumabHCA1680.1 mgE
            HCA168
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Human anti TrastuzumabHCA1770.1 mgE
            HCA177
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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