Substance P Antibody | NC1/34

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Substance P Antibody | NC1/34 gallery image 1

Published customer image:
Rat anti substance P antibody, clone NC1/34 (8450-0505) used for the detection of substance P expressing cells in tendon tissue.
Image caption:
Double stainings for PARs and substance P. A-D shows PARs [1]-[4] and E-H shows corresponding SP-staining; PAR-1 (A) is co-localised with SP-positive nerve fibres (E) (arrows). The same was seen for PAR-2 (nerve fascicle in B&F), PAR-3 (nerve fascicle in C&G) and PAR-4 (D&H, arrows show perivascular nerve fibres).

From: Christensen, J. Alfredson, H. and Andersson, G. "Protease-activated receptors in the Achilles tendon–a potential explanation for the excessive pain signalling in tendinopathy".
Mol Pain 11: 13. 2015.

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Rat antiSubstance P antibody, clone NC1/34 (8450-0505) used for the identification of substance P immunoreactive neurons in porcine dorsal root ganglia by immunofluorescence.
Image caption:
Representative images of substance P-positive (SP+) dorsal root ganglia (DRG)-urinary bladder-projecting neurons (UBPN) in control pigs. All images were taken separately from blue (a,d,g,j,m,p), green (b,e,h,k,n,r) and red (c,f,i,l,o,s) fluorescent channels; a–c One fast blue-positive (FB+) neuron (a, blue, arrow), which simultaneously contains SP (b, green, arrow) and pituitary adenylate cyclase activating peptide-PACAP (c, red, arrow). d–f One FB+ neuron (d, blue, arrow), which is simultaneously SP+ (e, green, arrow) and calcitonin gene-related peptide-positive (CGRP+, f, red, arrow). g–i Two FB+ neurons (g, blue, 1 short arrow, 1 long arrow), which are simultaneously SP+ (h, green, 1 short arrow) or SP-negative (h, green, 1 long arrow) and galanin-positive (GAL+, i, red, 1 short arrow) or GAL-negative (GAL-, i, red, 1 red arrow). j–l One FB+ neuron (j, blue, arrow), which simultaneously contains neuronal nitric oxide synthase-nNOS (k, green, arrow) and SP (l, red, arrow). m–o One FB+ neuron (m, blue, arrow), which is simultaneously calbindin-positive (CB+, n, green, arrow) and SP+ (o, red, arrow). p-s One FB+ neuron (p, blue, arrow), which simultaneously contains SP (r, green, arrow) and somatostatin-SOM (s, red, arrow). Bars 50 μm (a–s).

From: Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W. Botulinum Toxin Type A Induces Changes in the Chemical Coding of Substance P-ImmunoreactiveDorsal Root Ganglia Sensory Neurons Supplying the Porcine Urinary Bladder.
Toxins (Basel). 2015 Nov 16;7(11):4797-4816.

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Published customer image:
Rat antiSubstance P antibody, clone NC1/34 (8450-0505) used for the identification of substance P immunoreactive neurons in porcine dorsal root ganglia by immunofluorescence.
Image caption:
Representative images of substance P-positive (SP+) dorsal root ganglia (DRG)-urinary bladder-projecting neurons (UBPN) in BTX-treated animals. All images were taken separately from blue (a,d,g,j,m,p), green (b,e,h,k,n,r) and red (c,f,i,l,o,s) fluorescent channels; a–c one fast blue-positive (FB+) neuron (a, blue, arrow), which simultaneously contains SP (b, green, arrow) and pituitary adenylate cyclase activating peptide-PACAP (c, red, arrow). d–f One FB+ neuron (d, blue, arrow), which is simultaneously SP+ (e, green, arrow) and calcitonin gene-related peptide-positive (CGRP+, f, red, arrow). g–i One FB+ neuron (g, blue, arrow), which is simultaneously SP+ (h, green, arrow) and galanin-positive (GAL+, i, red, arrow). j–l One FB+ neuron (j, blue, arrow), which simultaneously contains neuronal nitric oxide synthase-nNOS (k, green, arrow) and SP (l, red, arrow). m–o One FB+ neuron (m, blue, arrow), which is simultaneously SP+ (n, green, arrow) and calbindin-negative (CB-, o, red, arrow). p-s One FB+ neuron (p, blue, arrow), which simultaneously contains SP (r, green, arrow) and somatostatin-SOM (s, red, arrow). Bars 50 μm (a–s).

From: Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W. Botulinum Toxin Type A Induces Changes in the Chemical Coding of Substance P-ImmunoreactiveDorsal Root Ganglia Sensory Neurons Supplying the Porcine Urinary Bladder.
Toxins (Basel). 2015 Nov 16;7(11):4797-4816.

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Substance P Antibody | NC1/34 gallery image 4

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Rat anti Substance P anitbody, clone NC1/34 (8450-0505) used for the evaluation of substance P expression on rabbit muscle nerve fasciles by immunofluorescence.
Image caption
Tachykinin-like immunoreactivity in nerve fascicles. Nerve fascicles and parts of nerve fascicles (N) of sections from gastrocnemius (A-C, E, F) and soleus (D) muscle specimens are shown. The sections were processed for demonstration of tachykinin-like immunoreaction (A-E). In (F) the reaction pattern after preabsorption with SP peptide is shown (the region in F corresponds to the region in E). The antibodies applied were sc-14104 (A-C, E) and 8450–0505 (D). The specimens were from the control group (A, B, E, F) and from the 6w group, non-exercised side, focal myositis area (C, D). There are immunoreactivities in nerve fascicles in the form of punctuate reactions (arrows A, C-E). These are grouped, as especially seen in (C), but also to some extent in (D). There are no reactions in the nerve fascicle in (B), which is built up of myelinated nerve fibers, and in the control section in (F). MF refers to muscle fibers. Magnification x200 (B, C), x315 (A, D, E, F).

From: Song Y, Stål PS, Yu JG, Forsgren S.
Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
BMC Musculoskelet Disord. 2013 Apr 12;14:134.

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Published customer image:
Rat anti Substance P anitbody, clone NC1/34 (8450-0505) used for the evaluation of substance P expression on rabbit muscle nerve fasciles by immunofluorescence.
Image caption
A,B. Tachykinin-like immunoreactivity in cells in inflammatory infiltrate of myositis area. Sections of a specimen from a soleus muscle specimen of the 6w group, non-exercised side. In (A), the section had been immunostained with 8450–0505, and in (B), the section was immunostained with 8450–0505 after preabsorption with tachykinin (SP) (larger area is covered in B than in A). Immunoreactivity is seen in cells in (A), but no reaction is visible in (B). Arrows point at cells of inflammatory infiltrate. The immunoreactions show partly a granular appearance (A). Magnification x315. c,d. Immunostaining with polyclonal and monoclonal tachykinin antibodies. A part of a focal myositis area of a section of a gastrocnemius muscle specimen from the 1w group, exercised side, double-stained for tachykinin using the sc-14104 (C) and 8450–0505 (D) antibodies, shown at low magnification. Note that the cells of an inflammatory infiltrate are immunolabelled in both (C) and (D) (arrows), whereas the wall of a small vessel is only immunostained in (C) (asterisks). Magnification x315.

From: Song Y, Stål PS, Yu JG, Forsgren S.
Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
BMC Musculoskelet Disord. 2013 Apr 12;14:134.

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Published customer image:
Rat anti substance P antibody, clone NC1/34 (8450-0505) used for the illustration of substance P expressing cells in porcine medulla oblongata by immubnofluorescence.
Image caption:
a, FB-labeled neurons (arrows) in the porcine control DMX. b, GAL-IR fibers (arrowheads) in the DMX. c, GAL-IR fibers (arrowheads) in close proximity of the FB-labeled somata (arrows) in the DMX. d, FB-labeled neurons (arrows) in the porcine control DMX. e, SP-IR fibers (arrowheads) in the DMX. f, SP-IR fibers (arrowheads) surrounded the FB-labeled somata (arrows) enabling direct contact. g, FB-labeled neurons (arrows) in the porcine control DMX. h, LENK-IR fibers (arrowheads) in the DMX. i, LENK-IR fibers (arrowheads) formed basket-like structures encircling FB-labeled neurons (arrows) in the DMX. j, FB-labeled neuron (arrow) in the porcine control DMX. k, CART-IR fibers (arrowheads) in the DMX. l, CART-IR fibers (arrowheads) in close proximity of the FB-positive perikaryon (arrow) in the DMX. Photographs of Fig. c, f, i, l were prepared by superimposition of single image

From: Gańko M, & Całka J.
Prolonged acetylsalicylic-acid-supplementation-induced gastritis affects the chemical coding of the stomach innervating vagal efferent neurons in the porcine dorsal motor vagal nucleus (DMX).
J Mol Neurosci. 2014;54(2):188-98.

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Published customer image:
Rat anti Substance P antibody, clone NC1/34 (8450-0505) used for the demonstration of neurons in porcine descending colon by immunofluorescence.
Image caption:
Representative images of co-localization of calcitonin gene-related peptide (CGRP) with other neuronal active substances in the neurons of myenteric plexus (I,II,III) and nerve fibers scattered in the circular muscle layer (IV) of the porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration. I—co-localization of CGRP with substance P (SP); II—co-localization of CGRP with galanin (GAL). Images are composites of merged images taken separately from green (CGRP) and red (other substances studied) fluorescent channels. Nervous structures, where CGRP co-localizes with other substances, are indicated by arrows.

From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.

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Substance P Antibody | NC1/34 gallery image 8

Published customer image:
Rat anti Substance P antibody, clone NC1/34 (8450-0505) used for the demonstration of neurons in porcine descending colon by immunofluorescence.
Image caption:
Representative images of co-localization of calcitonin gene-related peptide (CGRP) with other neuronal active substances in the neurons of the outer submucous plexus in the wall of porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration. I—co-localization of CGRP with substance P (SP); II—co-localization of CGRP with galanin (GAL); III—co-localization of CGRP with cocaine- and amphetamine- regulated transcript (CART) peptide. Images are composites of merged images taken separately from green (CGRP) and red (other substances studied) fluorescent channels. Neurons, where CGRP co-localizes with other substances are indicated by arrows.

From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.

Enlarge
Substance P Antibody | NC1/34 gallery image 9

Published customer image:
Rat anti Substance P antibody, clone NC1/34 (8450-0505) used for the demonstration of neurons in porcine descending colon by immunofluorescence.
Image caption:
Representative images of co-localization of calcitonin gene related peptide (CGRP) with other neuronal active substances in the neurons of inner submucous plexus (I,II,III) and nerve fibers scattered in submucous/mucous layer (IV) of the porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration. I—co-localization of CGRP with substance P (SP); II—co-localization of CGRP with galanin (GAL). Images are composites of merged images taken separately from green (CGRP) and red (other substances studied) fluorescent channels. Nervous structures, where CGRP co-localizes with other substances are indicated by arrows.

From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.

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  • Rat anti Substance P
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    NC1/34
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    8450-0505C, IF, P *datasheet pdfdatasheet pdf0.1 ml
    8450-0505
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti substance P antibody, clone NC1/34 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction.

      5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.
    • Intended Use
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MammalsExpected from Sequence
      Pigyes
      Pigeonyes
      Bichiryes
      Rabbityes
      Humanyes
      Chickenyes
      Chinchillayes
      Camelyes
      AmphibiaExpected from Sequence
      Cockroachyes
      Sheepyes
      Cowbirdyes
      Ratyes
      Spadefoot toadyes
      Homarus sp.yes
      Mudpuppyyes
      Lampreyyes
      Crayfishyes
      Zebrafishyes
      Bullfrogyes
      Bovineyes
      Guinea Pigyes
      Calanus sp.yes
      CrustaceaExpected from Sequence
      Cancer sp.yes
      Panilurus sp.yes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.05%Thiomersal
    • Immunogen
      Substance P conjugated to Bovine Serum Albumin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised Wistar rat were fused with cells of the NS1/1-Ag 4-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen1/1001/200
      Immunohistology - Paraffin(1)1/1001/200
      (1)
      This product does not require antigen retrieval prior to staining of formalin fixed, paraffin embedded sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Substance P Antibody Formats

    Formats Clone Applications Sizes available
    Substance P Antibody : S/N NC1/34 C, IF, P * 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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