Podcast Series: Fundamentals of Secondary Antibodies

Our Podcast Series

Understanding secondary detection is essential for getting the right result but it is often overlooked or neglected leading to poor results.

The podcast series include:

  • Tips for choosing secondary antibodies
  • Considerations for multiplexing experiments
  • Does binding site matter?
  • Understanding cross-adsorbed antibodies

Fundamentals of Secondary Antibodies

Listen to our secondary antibody podcast series to improve your experiments by learning how to choose and use these critical detection reagents
 
  • Tips for choosing secondary antibodies This podcast covers the main factors that impact which secondary antibodies you should use in your experiments for the best results.
  • Understanding cross-adsorbed antibodiesCould cross-adsorbed secondary antibodies reduce your background and improve your results? Learn about cross-adsorption and how it removes undesirable cross-reactivity.
  • Does binding site matter?Do you know where on the primary antibody your secondary antibody binds? Learn why it can i mpact your results and how to choose an appropriate secondary antibody.
  • Considerations for multiplexing experimentsThis podcast highlights five important things to consider when you are selecting secondary antibodies for multiplexing experiments.

Popular Resources

Secondary Antibody Guide

Secondary Antibody Guide

Download or request a free printed copy of Bio-Rad’s new secondary antibody guide. It is easy-to-read and practical, explaining step-by-step what you need to know, what you need to do, and how to do it.
Fundamentals of Secondary Antibodies

Fundamentals of Secondary Antibodies

Listen to the secondary antibody podcast series to improve your experiments by learning how to choose and use these critical detection reagents.
Using Secondary Reagents in Flow Cytometry with Confidence

Using Secondary Reagents in Flow Cytometry with Confidence

Directly conjugated antibodies are preferred for flow cytometry, but secondary antibodies provide a valuable alternative when conjugation is not feasible.