Rituximab Antibody | AbD02844

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  • Human anti Rituximab
  • Human anti Rituximab:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-rituximab antibody is a recombinant, neutralizing, anti-idiotypic antibody in bivalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It has been validated as detection antibody in PK bridging ELISA format.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD02844
    • Isotype
      HuCAL Fab bivalent
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA062Edatasheet pdfdatasheet pdf0.1 mg
      HCA062
      HCA062PEdatasheet pdfdatasheet pdf0.1 mg
      HCA062P
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti Rituximab antibody, clone AbD02844 recognizes the idiotypic determinants expressed by the rituximab humanised monoclonal antibody. Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. Human anti Rituximab antibody, clone AbD02844 does not recognize other CD20 antibodies.
      • Intended Use
      • Product Form
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain and is conjugated to horseradish peroxidase.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
        HRP Stabiliser (BUF052A)
      • Immunogen
        Rituximab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=10 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5mg/ml
        Antibody concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC. DO NOT FREEZE.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
        Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
        MabThera ® is a registered trademark of Roche in Europe
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
        Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
        MabThera ® is a registered trademark of Roche in Europe.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA1/500
      • Application NameYesNoMin DilutionMax Dilution
        ELISA1/2501/1000

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        In its HRP conjugated format (HCA062P ), this product may be used as a detection reagent in a sandwich ELISA.
        Protocol:PK bridging ELISA to measure free drug.
      • ELISA
        This product may be used as a detection reagent in a sandwich ELISA.
        Protocol: PK bridging ELISA to measure free drug.
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Rituximab Antibody Formats

      Formats Clone Applications Sizes available
      Rituximab Antibody : HRP AbD02844 E 0.1 mg
      Rituximab Antibody : Purified AbD02844 E 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
        MCA2200P
        Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
        0500-0099
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            MCA2260F
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA1860.1 mgE
            HCA186
            Rat anti RituximabMCA22600.2 mgE, F
            MCA2260
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            MCA2260F
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA1860.1 mgE
            HCA186
            Rat anti RituximabMCA22600.2 mgE, F
            MCA2260
            AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
            BUF052A
            TMB Signal+BUF054A100 mlE
            BUF054A
            TMB CoreBUF056A100 mlE
            BUF056A
            TMB Core PrestainedBUF057A100 mlE
            BUF057A
            TMB Core+BUF062A100 mlE
            BUF062A
            AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
            BUF052C
            AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
            BUF052B

            Recommended Positive Controls

              Histology Controls

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