Proteus Protein Purification Spin Kits

Purification Kits Binding Affinities for Protein A and G
Technology Advantage Frequently Asked Questions
Antibody Purification Ordering Information
Endotoxin Removal  
UF Spinner - Instructions for Use  

Purification Kits

Proteus purification kits use a unique technology that combines protein separation by gravity flow with the speed and ease-of-use of spin columns. A range of kits incorporating Protein A or Protein G is available for the purification of antibodies.

  • MINI Kits for purification of up to 1 mg of protein in 15 minutes
  • MIDI Kits for purification of up to 20 mg of protein in 60 minutes
  • Kits include pre-packed columns and buffers
  • Comprehensive handbook

Technology Advantage

The unique technology incorporated in the Proteus kits uses back pressure to enable a steady and controlled flow of sample and buffer through the affinity resin column during centrifugation. This results in higher antibody yields and improved purification results when compared to similar gravity flow and spin systems. The columns are supplied pre-packed and ready-to-use with buffers included in the kits, to ensure consistency of results.


Antibody Purification
 

Small Scale Purification: Proteus Protein A and G MINI Kits

  • From sample to pure antibody in under 20 minutes
  • Ideal for small-scale antibody purification
  • Ready-to-use buffers for 48 mini preps

Proteus MINI Protein A & G Antibody Purification Kits allow you to rapidly purify typically 1 mg of antibody in your standard bench microfuge. They are ideal for:

(a) Screening antibody expression levels.
(b) Purification of antibodies for solution-state immunoassays, immunohistochemical and immunofluorescence studies, Western blotting and immunoprecipitation experiments.

Large-scale Purification: Proteus Protein A and G MIDI Kits

  • From sample to pure antibody in under 60 minutes
  • High yields of functional antibodies
  • Small elution volumes for high antibody concentrations

Proteus MIDI Protein A & G Antibody Purification Kits are ideal for semi-preparative purification of typically 20 mg of concentrated monoclonal and polyclonal antibodies for all downstream applications


Endotoxin Removal

Proteus Endotoxin Removal Kits are designed for easy and effective removal of endotoxins from antibodies, recombinant proteins, and viral vectors. 

  • Rapid removal of endotoxin in less than 1 hour
  • High capacity kits with a binding capacity of 450,000 EU/ml
  • MINI and MIDI formats depending on sample size and endotoxin loads

UF Spinner - Instructions for Use