to Protein A and Protein G
Depending on their host species and isotype, antibodies have different binding affinities to protein A and protein G. These properties are especially critical when deciding whether to use protein A or protein G coupled agarose, sepharose or magnetic beads in your immunoprecipitation experiments. While the binding affinity differences to protein A or protein G for a human IgG1 antibody may be minimal, for other antibodies the differences are severe; for example rat Ig2a does not bind to protein A but has a strong binding affinity to protein G.
In addition to immunoprecipitation experiments, the choice between protein A and protein G is also critical when selecting the best antibody purification method or kit.
Antibody Host Species, Isotype
Guinea Pig IgG
**** = Strong binding affinity
*** = Moderate binding affinity
** = Weak binding affinity
* = Very weak binding affinity
x = No binding affinity
Table and legend were adapted from here
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