Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Bio-Rad-Antibodies.com relies on third-party cookies to show you pricing, allow you to order online, and connect you to My Bio-Rad. Please amend your browser settings to enable third-party cookies and access this website’s full functionality.
Click here to find out how
to Protein A and Protein G
Depending on their host species and isotype, antibodies have different binding affinities to protein A and protein G. These properties are especially critical when deciding whether to use protein A or protein G coupled agarose, sepharose or magnetic beads in your immunoprecipitation experiments. While the binding affinity differences to protein A or protein G for a human IgG1 antibody may be minimal, for other antibodies the differences are severe; for example rat Ig2a does not bind to protein A but has a strong binding affinity to protein G.
In addition to immunoprecipitation experiments, the choice between protein A and protein G is also critical when selecting the best antibody purification method or kit.
Antibody Host Species, Isotype
Guinea Pig IgG
**** = Strong binding affinity
*** = Moderate binding affinity
** = Weak binding affinity
* = Very weak binding affinity
x = No binding affinity
Table and legend were adapted from here