Human IgM-FcSPYCATCHER3
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EP SC ResetHuman IgM-FcSpyCatcher3
- Product Type
- Recombinant Protein
- Specificity
- Human IgM-FcSPYCATCHER3
Human IgM-FcSpyCatcher3 (hIgM-FcSpyC3) contains a SpyCatcher3 (Keeble et al. 2019) fused to the hinge region, CH2 and CH3 domains of human IgM, resulting in a 102.4 kDa protein for each disulfide-bridge IgM monomer. Analysis of the hIgM-FcSpyCatcher3 (TZC036) shows that it spontanously forms pentameric and hexameric IgMs and that it does not contain any J chain. Each SpyCatcher3 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity. The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2+/Mg2+ not needed) and in the presence of detergents. The reaction also occurs inside cells (in vivo). Since the speed of the coupling reaction is concentration dependent, it is recommended to use hIgM-FcCatcher3 undiluted. Human IgM-FcSpyCatcher3 has been successfully tested in ELISA, Western-Blotting and precipitation assays and is compatible with Bio-Rad's recombinant HuCAL antibodies with a SpyTag2 at the C-terminus of the heavy chain. Download hIgM-FcSpyCatcher3 coupling protocol View all available Catchers |
- Target Species
- Protein/peptide tag
- Product Form
- FcSpyCatcher3 purified recombinant protein, fused to the hinge region, CH2, and CH3 of human IgM - liquid
- Preparation
- Recombinant protein expressed in mammalian cell line and purified by affinity chromatography
- Source
- CHO-S
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Purity
- ≥90% determined by SDS-PAGE under reducing conditions and visualized by coomassie blue staining
- Approx. Protein Concentrations
- 40 µM (4.1 mg/ml)
- Protein Molecular Weight
- Predicted molecular weight of the pentameric form 512 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunoassay |
- Instructions For Use
-
TZC036
View FcSpyCatcher Coupling Protocol
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
SpyTag3 Peptide | BLP086 | IY | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | SpyTag3 Peptide |
Antibody Characterization Reference
-
Hentrich, C. et al. (2021) Periplasmic expression of SpyTagged antibody fragments enables rapid modular antibody assembly.
Cell Chem Biol. 28 (6): 813-824.e6. -
Zakeri, B. et al. (2012) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
Proc Natl Acad Sci U S A. 109 (12): E690-7. -
Keeble, A.H. et al. (2019) Approaching infinite affinity through engineering of peptide-protein interaction.
Proc Natl Acad Sci U S A. 10: 116 (52): 26523-33.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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