MIP-2

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Recombinant Mouse MIP-2

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP55 E FN WB 20 µg
Mouse macrophage inflammatory protein-2 (MIP-2) is a 7.8 kDa chemokine, also known as CXCL2. MIP-2 is chemotactic for neutrophils and is expressed by macrophages and epidermal langerhans cells.

Product Details

Target Species
Mouse
Product Form
Purified Recombinant Protein - lyophilized
Reconstitution
Reconstitute with 0.2 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Recombinant protein expressed in E. coli
Buffer Solution
10mM Acetic Acid
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Protein Molecular Weight
7.8 kDa (73 amino acid residues)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution

More Information

UniProt
P10889 Related reagents
Entrez Gene
Cxcl2 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of MIP-2

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1.0 10.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PMP55 may be used as a standard for ELISA applications with either AAM48 or AAM48B.
Western Blotting
PMP55 may be used as a positive control for western blotting applications with AAM48 or AAM48B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Mouse MIP-2 AAM48 0.1 mg C E FN P* WB
Rabbit anti Mouse MIP-2:Biotin AAM48B 50 µg C E P* WB