MIP-2

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Recombinant Mouse MIP-2

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PMP55	E FN WB			20 µg

Summary
Useful Reagents

Mouse macrophage inflammatory protein-2 (MIP-2) is a 7.8kD chemokine, also known as CXCL2. MIP-2 is chemotactic for neutrophils and is expressed by macrophages and epidermal langerhans cells.

Product Details

Target Species: Mouse
Product Form: Purified Recombinant Protein - lyophilised
Reconstitution: Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation: Recombinant protein expressed in E. coli.
Buffer Solution: 10mM Acetic Acid
Preservative Stabilisers: None present
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.1mg/ml after reconstitution
Protein Molecular Weight: 7.8kD (73 amino acid residues)
Endotoxin Level: <0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

More Information

UniProt: P10889 Related reagents
Entrez Gene: Cxcl2 Related reagents
GO Terms: GO:0005615 extracellular space; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0008009 chemokine activity
Regulatory: For research purposes only

Applications of MIP-2

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	1.0	10.0ng/ml
Western Blotting	1.5	3.0ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PMP55 may be used as a standard for ELISA applications with either AAM48 or AAM48B.
Western Blotting: PMP55 may be used as a positive control for western blotting applications with AAM48 or AAM48B.

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Mouse MIP-2	AAM48	0.1 mg	C E FN P* WB
Rabbit anti Mouse MIP-2:Biotin	AAM48B	50 µg	C E P* WB

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