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MCP-1

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Recombinant Mouse MCP-1

Product Type
Recombinant Protein
Specificity
MCP-1

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Recombinant mouse MCP-1 is 13.8 kDa peptide corresponding to full-length mature mouse monocyte chemotactic protein 1. MCP-1, otherwise known as CCL2 or JE, is a secreted chemokine involved in the recruitment of monocytes to sites of injury or inflammation (Lu, B. et al., 1998).

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein prepared from E. coli.
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Protein Molecular Weight
13.8kD (125 amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1.0 20ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
This product may be used as a standard for ELISA applications with our purified mouse MCP-1 antibody (AAM43).

References for MCP-1

  1. Ren, Z. et al. (2019) Minocycline attenuates ethanol-induced cell death and microglial activation in the developing spinal cord.
    Alcohol. 79: 25-35.

Synonyms
CCL2
UniProt
P10148
Entrez Gene
Ccl2
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010759 positive regulation of macrophage chemotaxis
GO:0016525 negative regulation of angiogenesis
GO:0090265 positive regulation of immune complex clearance by monocytes and macrophages
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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