Interleukin-1 beta (IL-1 beta), is a 17.5 kDa proinflammatory, cell-associated cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1 beta promotes the proliferation of memory T cells and cytokine production. It attenuates regulatory T cell function and enables CD4+CD25- autoreactive effectors to avoid suppression. IL-1 beta signals downstream of myc and is required for angiogenesis and invasiveness of tumour cells. IL-1 beta is directly involved in neuronal injury in neurodegenerative disorders, and stimulates mitogenic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandin from synovial cells.
- Target Species
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified recombinant murine IL-1 beta, expressed in E. coli
- Preservative Stabilisers
- None present
- The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.002ng/ml, corresponding to a specific activity of > 5x108 units/mg.
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.1mg/ml after reconstitution
- Molecular Weight
- 17.5kD (153 amino acid residues)
- Endotoxin Level
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 3 months from date of reconstitution.
- P10749 Related reagents
- Entrez Gene
- Il1b Related reagents
- GO Terms
- GO:0001660 fever generation
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0008083 growth factor activity
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010829 negative regulation of glucose transport
- GO:0030593 neutrophil chemotaxis
- GO:0031622 positive regulation of fever generation
- GO:0032308 positive regulation of prostaglandin secretion
- GO:0032496 response to lipopolysaccharide
- GO:0032755 positive regulation of interleukin-6 production
- GO:0033198 response to ATP
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0045766 positive regulation of angiogenesis
- GO:0046330 positive regulation of JNK cascade
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0050996 positive regulation of lipid catabolic process
- GO:0051781 positive regulation of cell division
- GO:0070164 negative regulation of adiponectin secretion
- For research purposes only
Applications of IL-1 Beta
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Rabbit anti Mouse Interleukin-1 beta:Biotin||AAM13B||50 µg||E WB|
|Rabbit anti Mouse Interleukin-1 beta||AAM13G||0.1 mg||C E FN IF WB|
Product Specific References
References for IL-1 Beta
Allan, S. et al. (2005) Interleukin-1 and neuronal injury.
Nature Reviews Immunology 5: 629-640.