IL-1 Beta

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Recombinant Mouse Interleukin-1 beta

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP71 E FN WB 10 µg
Interleukin-1 beta (IL-1 beta), is a 17.5 kDa proinflammatory, cell-associated cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1 beta promotes the proliferation of memory T cells and cytokine production. It attenuates regulatory T cell function and enables CD4+CD25- autoreactive effectors to avoid suppression. IL-1 beta signals downstream of myc and is required for angiogenesis and invasiveness of tumour cells. IL-1 beta is directly involved in neuronal injury in neurodegenerative disorders, and stimulates mitogenic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandin from synovial cells.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant murine IL-1 beta, expressed in E. coli
Preservative Stabilisers
None present
Activity
The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.002ng/ml, corresponding to a specific activity of > 5x108 units/mg.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Molecular Weight
17.5kD (153 amino acid residues)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P10749 Related reagents
Entrez Gene
Il1b Related reagents
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0008083 growth factor activity
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010829 negative regulation of glucose transport
GO:0030593 neutrophil chemotaxis
GO:0031622 positive regulation of fever generation
GO:0032308 positive regulation of prostaglandin secretion
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0033198 response to ATP
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043407 negative regulation of MAP kinase activity
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0046330 positive regulation of JNK cascade
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050996 positive regulation of lipid catabolic process
GO:0051781 positive regulation of cell division
GO:0070164 negative regulation of adiponectin secretion
Regulatory
For research purposes only

Applications of IL-1 Beta

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PMP71 may be used as an ELISA standard for our AAM13G and AAM13B antibodies.
Western Blotting
PMP71 may be used as a postive control for Western blotting applications with our AAM13G and AAM13B antibodies.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Mouse Interleukin-1 beta:Biotin AAM13B 50 µg E WB
Rabbit anti Mouse Interleukin-1 beta AAM13G 0.1 mg C E FN IF WB

Product Specific References

References for IL-1 Beta

  1. Allan, S. et al. (2005) Interleukin-1 and neuronal injury.
    Nature Reviews Immunology 5: 629-640.