IgM | MADNP-5

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Mouse IgM Kappa

Product Type: Purified Protein
Clone: MADNP-5
Isotype: IgM

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PMP52 Datasheet	E SDS	1 mg	Log in		Get Quote

Product Details

Target Species

Mouse

Product Form

Purified monoclonal IgM - liquid

Preparation

Purified IgM prepared by affinity chromatography from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1%	Sodium Azide

Purity

Single band by agarose electrophoresis and immunoelectrophoresis.

Approx. Protein Concentrations

IgM concentration 1.0 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P01837; P01872; P01873
Entrez Gene: Igk-C; Ighm; Ighm
GO Terms: GO:0003823 antigen binding; GO:0000187 activation of MAPK activity; GO:0005515 protein binding; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0004888 transmembrane receptor activity; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0019882 antigen processing and presentation; GO:0030890 positive regulation of B cell proliferation; GO:0042571 immunoglobulin complex, circulating; GO:0043410 positive regulation of MAPKKK cascade; GO:0045022 early endosome to late endosome transport; GO:0045807 positive regulation of endocytosis; GO:0048471 perinuclear region of cytoplasm; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050853 B cell receptor signaling pathway; GO:0016021 integral to membrane
Regulatory: For research purposes only

Applications of IgM

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1.25ug/ml

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgM

Hayer, S. et al. (2008) B-cell infiltrates induce endosteal bone formation in inflammatory arthritis.
J Bone Miner Res. 23: 1650-60.
Rohrer, J.W. et al. (2006) Identification of oncofetal antigen/immature laminin receptor protein epitopes that activate BALB/c mouse OFA/iLRP-specific effector and regulatory T cell clones.
J Immunol. 176: 2844-56.

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