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IgM | MADNP-5

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Mouse IgM Kappa, clone MADNP-5 thumbnail image 1
Mouse IgM Kappa, clone MADNP-5 gallery image 1
Published customer image:
Purified murine IgM (PMP52) used as a standard in an ELISA for the measurement of IgM production by peritoneal cells in vitro.
Image caption:
Loss of SIRPα signaling promotes LPS-induced CD11b/CD18 integrin-dependent B1 cell efflux from the peritoneal cavity. (D) Blockade of CD11b/CD18 or CD11a/CD18 integrins has no effect on IgM antibody production by peritoneal cavity B1 cells upon LPS stimulation.

From: Franke K, Pillai SY, Hoogenboezem M, Gijbels MJJ, Matlung HL, Geissler J, Olsman H, Pottgens C, van Gorp PJ, Ozsvar-Kozma M, Saito Y, Matozaki T, Kuijpers TW, Hendriks RW, Kraal G, Binder CJ, de Winther MPJ, van den Berg TK.
SIRPα on Mouse B1 Cells Restricts Lymphoid Tissue Migration and Natural Antibody Production.
Front Immunol. 2020 Oct 9;11:570963.
doi: 10.3389/fimmu.2020.570963..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse IgM Kappa

Product Type
Purified Protein
Clone
MADNP-5
Isotype
IgM
Specificity
IgM
Region
KAPPA

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Product Code Applications Pack Size List Price Your Price Qty
PMP52
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader

Purified Mouse IgM is a preparation of purified murine IgM derived from the murine MADNP-5 hybridoma.

Target Species
Mouse
Product Form
Purified monoclonal IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Purity
Single band by agarose electrophoresis and immunoelectrophoresis.
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.25ug/ml
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.

References for IgM

  1. Hayer, S. et al. (2008) B-cell infiltrates induce endosteal bone formation in inflammatory arthritis.
    J Bone Miner Res. 23: 1650-60.
  2. Rohrer, J.W. et al. (2006) Identification of oncofetal antigen/immature laminin receptor protein epitopes that activate BALB/c mouse OFA/iLRP-specific effector and regulatory T cell clones.
    J Immunol. 176: 2844-56.
  3. Franke, K. et al. (2020) SIRPα on Mouse B1 Cells Restricts Lymphoid Tissue Migration and Natural Antibody Production.
    Front Immunol. 11: 570963.

RRID
AB_324175
UniProt
P01837
P01872
P01873
Entrez Gene
Igk-C
Ighm
Ighm
GO Terms
GO:0003823 antigen binding
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane

PMP52

147622 162550 166114 300415

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