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IgG1 | MADNP-1

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Mouse IgG1 Kappa

Product Type
Purified Protein
Clone
MADNP-1
Isotype
IgG1
Specificity
IgG1
Region
KAPPA

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Mouse IgG1 Kappa

Target Species
Mouse
Product Form
Purified monoclonal IgG1 - liquid
Preparation
Purified IgG1 prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Purity
Single band by agarose electrophoresis and immunoelectrophoresis.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.6 ug/ml 1.25 ug/ml
Gel Super Shift Assays
Where this proetin has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.

References for IgG1

  1. Kiesler, P. et al. (2009) An allergy-associated polymorphism in a novel regulatory element enhances IL13 expression.
    Hum Mol Genet. 18: 4513-20.
  2. Brown, J.K. et al. (2011) Human Fallopian tube epithelium constitutively expresses integrin endometrial receptivity markers: no evidence for a tubal implantation window.
    Mol Hum Reprod. 18: 111-20.
  3. Laffont, S. et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
    J Exp Med. 214 (6): 1581-92.

Gel Super Shift Assays

Immunofluorescence

Immunohistology - Paraffin

RRID
AB_324554
UniProt
P01837
P01869
P01868
Entrez Gene
Igk-C
Ighg1
Ighg1
GO Terms
GO:0003823 antigen binding
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0005886 plasma membrane
GO:0016021 integral to membrane

PMP48

154929 180220

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