SDF-1 Alpha

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Recombinant Human SDF-1 Alpha

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP122
Datasheet
E FN WB
SDS
10 µg loader

Stromal cell derived factor 1 alpha (SDF-1 alpha) belongs to the C-X-C family of human chemokines.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein prepared in E.coli
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
8.0 kDa (68 amino acid sequence)
Endotoxin Level
< 0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P48061
Entrez Gene
CXCL12
GO Terms
GO:0009615 response to virus
GO:0007155 cell adhesion
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008009 chemokine activity
GO:0008015 blood circulation
GO:0008064 regulation of actin polymerization or depolymerization
GO:0008083 growth factor activity
GO:0090026 positive regulation of monocyte chemotaxis
Regulatory
For research purposes only

Applications of SDF-1 Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 20ng/ml 80ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP122 may be used in ELISA applications with either AHP794 or AHP794B.
Western Blotting
PHP122 may be used under reducing or non-reducing western blotting conditions with either AHP794 or AHP794B.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Human SDF-1 Alpha AHP794 E FN P* WB 0.1 mg loader
Rabbit anti Human SDF-1 Alpha:Biotin AHP794B E WB 50 µg loader