Human IgG1 | AbD18705il

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Recombinant Human IgG1 Kappa Allotype G1m17,1

Product Type
Recombinant Protein
Clone
AbD18705il
Isotype
IgG1 Kappa
Product Code Applications Pack Size List Price Quantity
HCA319
Datasheet
E
MSDS
0.1 mg loader

Recombinant Human IgG1 kappa allotype G1m17,1, clone AbD18705il is a recombinant human antibody with a kappa light chain, and heavy chain belonging to the G1m17,1 allotype, also designated G1m(z,a).

This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human IgG1 antibodies of the same isotype or allotype. It is recommended that this reagent is used at the same concentration as the test reagent.

The main allelic forms of IgG1 are G1m(z,a), G1m(f), and G1m(f,a). The G1m(z,a) (or G1m17,1) allele is found in Caucasoid, Negroid and Mongoloid populations, whereas the G1m3 allele is only found in Caucasoid; for further details on IgG1 allotypes see IgG subclasses and allotypes: from structure to effector functions, and IMGT Repertoire (IG and TR) Allotypes: Human (Homo sapiens) IGHC.

The following publication reviews the serologically defined human IgG allotypes and considers the potential for allotype differences to contribute to or potentiate immunogenicity; Human immunoglobulin allotypes, possible implications for immunogenicity

Our range of IgG1 controls also includes Recombinant Human IgG1 kappa allotype G1m3, and the corresponding allotypes for IgG1 lambda.

View a summary of all recombinant fully human immunoglobulin isotype controls

Product Details

Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgG1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Human IgG1 Lambda Allotype G1m3 HCA049 E 0.1 mg loader
Recombinant Human IgG1 Kappa Allotype G1m3 HCA192 E 0.1 mg loader
Recombinant Human IgG1 Lambda Allotype G1m17,1 HCA318 E 0.1 mg loader