Human IgG1 | AbD00264il

Recombinant Human IgG1 Lambda Allotype G1m17,1

Product Type
Recombinant Protein
Clone
AbD00264il
Isotype
IgG1 Lambda
Specificity
Human IgG1
Region
LAMBDA ALLOTYPE G1m17,1

Product Code Applications Pack Size List Price Your Price Qty
HCA318
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E 0.1 mg loader
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Recombinant Human IgG1 lambda allotype G1m17,1 clone AbD00264il is a recombinant human antibody with a lambda light chain, and heavy chain belonging to the G1m17,1 allotype, also designated G1m(z,a).

This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human IgG1 antibodies of the same isotype or allotype. It is recommended that this reagent is used at the same concentration as the test reagent.

The main allelic forms of IgG1 are G1m(z,a), G1m(f), and G1m(f,a). The G1m(z,a) (or G1m17,1) allele is found in Caucasoid, Negroid and Mongoloid populations, whereas the G1m3 allele is only found in Caucasoid; for further details on IgG1 allotypes see IgG subclasses and allotypes: from structure to effector functions, and IMGT Repertoire (IG and TR) Allotypes: Human (Homo sapiens) IGHC.

The following publication reviews the serologically defined human IgG allotypes and considers the potential for allotype differences to contribute to or potentiate immunogenicity; Human immunoglobulin allotypes, possible implications for immunogenicity

Our range of IgG1 controls also includes Recombinant Human IgG1 lambda allotype G1m3, and the corresponding allotypes for IgG1 kappa.

View a summary of all recombinant fully human immunoglobulin isotype controls

Product Form
Human IgG1 antibody selected from the HuCAL GOLD phage display library and expressed in a human cell line, HKB-11 - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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Description Recombinant Human IgG1 Kappa Allotype G1m3
Recombinant Human IgG1 Kappa Allotype G1m17,1 HCA319 E 0.1 mg loader
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Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
View more products with HUMAN IGG1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"