IFN Gamma

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Recombinant Human Interferon Gamma

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP050 Datasheet	E FN WB SDS	0.1 mg	Log in		Get Quote
PHP050A Datasheet	E FN WB SDS	20 µg	Log in		Get Quote

IFN-gamma is a lymphoid factor with anti viral activity and macrophage and NK cell stimulating properties.

Product Details

Target Species: Human
Product Form: Recombinant protein produced in E. coli - lyophilized.
Reconstitution: Pack Size: 0.1 mg
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Pack Size: 20 µg
Reconstitute with 20ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution: Pack Size: 0.1 mg
Phosphate buffered saline

Pack Size: 20 µg
Sodium Phosphate
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: > 2 x 10⁷ units/mg
Purity: >98% by SDS PAGE and HPLC
Approx. Protein Concentrations: Pack Size: 0.1 mg
Total protein concentration 1.0 mg/ml after reconstitution

Pack Size: 20 µg
1.0 mg/ml after reconstitution
Protein Molecular Weight: 16.7kD (143 Amino Acid Sequence)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Pack Size: 0.1 mg
Prior to reconstitution store at -20^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 20 µg
Prior to reconstitution store at -20^oC.

Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P01579
Entrez Gene: IFNG
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0002053 positive regulation of mesenchymal cell proliferation; GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; GO:0009615 response to virus; GO:0005125 cytokine activity; GO:0005133 interferon-gamma receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0007050 cell cycle arrest; GO:0050796 regulation of insulin secretion; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0032735 positive regulation of interleukin-12 production; GO:0032747 positive regulation of interleukin-23 production; GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein; GO:0034393 positive regulation of smooth muscle cell apoptosis; GO:0042508 tyrosine phosphorylation of Stat1 protein; GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045672 positive regulation of osteoclast differentiation; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051712 positive regulation of killing of cells of other organism; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060334 regulation of interferon-gamma-mediated signaling pathway; GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity; GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process; GO:0060557 positive regulation of vitamin D biosynthetic process; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
Regulatory: For research purposes only

Applications of IFN Gamma

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	0.1	10ng/ml
Western Blotting	1.5	3.0ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate positive/negative controls.

ELISA: PHP050 may be used in ELISA applications with either AHP775 or AHP775B.
Western Blotting: PHP050 may be used under reducing or non-reducing western blotting conditions with either AHP775 or AHP775B.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Human Interferon Gamma	AHP775	E FN P* WB	0.1 mg	Log in		Get Quote
Rabbit anti Human Interferon Gamma:Biotin	AHP775B	E WB	50 µg	Log in		Get Quote

Application Based External Images

Functional Assays

Product Specific References

References for IFN Gamma

Grisar, J. et al. (2001) Phenotypic characteristics of human monocytes undergoing transendothelial migration.
Arthritis Res. 3 (2): 127-32.
Fros, J.J. et al. (2010) Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling.
J Virol. 84 (20): 10877-87.
Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
Invest Ophthalmol Vis Sci. 52: 5278-86.
Wienken, C.J. et al. (2010) Protein-binding assays in biological liquids using microscale thermophoresis.
Nat Commun. 1: 100.
Bakmiwewa, S.M. et al. (2016) Synergistic induction of CXCL10 by interferon-gamma and lymphotoxin-alpha in astrocytes: Possible role in cerebral malaria.
Cytokine. 78: 79-86.

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