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IFN Gamma

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Recombinant Human interferon gamma (PHP050) used to treat human fibroblasts in vitro prior to infection with Toxoplasma
Image caption:
Lipidomic analyses on Δgra57, Δgra70, and Δgra71 using GC-MS-based approaches. (A) Lipidomics analysis comparing relative abundance of cholesterol over total fatty acids in WT and indicated knockout and complemented parasites grown in naive or IFNγ-stimulated HFFs. Indicated are means ± SEM (n = 2 to 4). P values were calculated using 2-way ANOVA with Sidak’s multiple-comparison test. (B) Fatty acid composition in Mol %. P values were calculated with a mixed-effects model with Sidak’s multiple-comparison test. n = 6 to 8 for WT and Δgra70, n = 2 for Δgra57, Δgra71, and Δgra70+GRA70. *, P <0.05; **, P <0.01; ***, P <0.001.

From: Krishnamurthy S, Maru P, Wang Y, Bitew MA, Mukhopadhyay D, Yamaryo-Botté Y, Paredes-Santos TC, Sangaré LO, Swale C, Botté CY, Saeij JPJ.
CRISPR Screens Identify Toxoplasma Genes That Determine Parasite Fitness in Interferon Gamma-Stimulated Human Cells.
mBio. 2023 Apr 25;14(2):e0006023.
doi: 10.1128/mbio.00060-23.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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FN

Recombinant Human Interferon Gamma

Product Type
Recombinant Protein
Specificity
IFN Gamma

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Product Code Applications Pack Size List Price Your Price Qty
PHP050
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
List Price Your Price
loader
PHP050A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 20 µg loader
List Price Your Price
loader

IFN-gamma is a lymphoid factor with anti viral activity and macrophage and NK cell stimulating properties.

Target Species
Human
Product Form
Recombinant protein produced in E. coli - lyophilized.
Reconstitution
Pack Size: 0.1 mg
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Pack Size: 20 µg
Reconstitute with 20ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Source
E.coli
Buffer Solution
Sodium Phosphate
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
> 2 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Pack Size: 0.1 mg
Total protein concentration 1.0 mg/ml after reconstitution
Pack Size: 20 µg
1.0 mg/ml after reconstitution
Protein Molecular Weight
16.7kD (143 Amino Acid Sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Pack Size: 0.1 mg
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 20 µg
Prior to reconstitution store at -20oC.

Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 10ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate positive/negative controls.
ELISA
PHP050 may be used in ELISA applications with either AHP775 or AHP775B.
Western Blotting
PHP050 may be used under reducing or non-reducing western blotting conditions with either AHP775 or AHP775B.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Human Interferon Gamma AHP775 E FN P * WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit anti Human Interferon Gamma
Rabbit anti Human Interferon Gamma:Biotin AHP775B E WB 50 µg loader
List Price Your Price
loader
Description Rabbit anti Human Interferon Gamma:Biotin

References for IFN Gamma

  1. Grisar, J. et al. (2001) Phenotypic characteristics of human monocytes undergoing transendothelial migration.
    Arthritis Res. 3 (2): 127-32.
  2. Fros, J.J. et al. (2010) Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling.
    J Virol. 84 (20): 10877-87.
  3. Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
    Invest Ophthalmol Vis Sci. 52: 5278-86.
  4. Wienken, C.J. et al. (2010) Protein-binding assays in biological liquids using microscale thermophoresis.
    Nat Commun. 1: 100.
  5. Bakmiwewa, S.M. et al. (2016) Synergistic induction of CXCL10 by interferon-gamma and lymphotoxin-alpha in astrocytes: Possible role in cerebral malaria.
    Cytokine. 78: 79-86.
  6. Krishnamurthy, S. et al. (2023) CRISPR Screens Identify Toxoplasma Genes That Determine Parasite Fitness in Interferon Gamma-Stimulated Human Cells.
    mBio. 14 (2): e0006023.

Functional Assays

Synonyms
Interferon Gamma
UniProt
P01579
Entrez Gene
IFNG
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0050796 regulation of insulin secretion
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032735 positive regulation of interleukin-12 production
GO:0032747 positive regulation of interleukin-23 production
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042508 tyrosine phosphorylation of Stat1 protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051712 positive regulation of killing of cells of other organism
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation

PHP050

152893 162284 162668

PHP050A

155292 157573 162285 164958

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