IFN Gamma

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Recombinant Human Interferon Gamma

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP050A E FN WB 20 µg
PHP050 E FN WB 0.1 mg
IFN-gamma is a lymphoid factor with anti viral activity and macrophage and NK cell stimulating properties.

Product Details

Target Species
Human
Product Form
Recombinant protein produced in E. coli - lyophilised.
Reconstitution
Pack Size: 20 µg
Reconstitute with 20ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Pack Size: 0.1 mg
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Pack Size: 20 µg
Sodium Phosphate
Pack Size: 0.1 mg
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
> 2 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Pack Size: 20 µg
1.0 mg/ml after reconstitution
Pack Size: 0.1 mg
Total protein concentration 1.0mg/ml after reconstitution.
Protein Molecular Weight
16.7kD (143 Amino Acid Sequence)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Pack Size: 20 µg
Prior to reconstitution store at -20oC.

Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.1 mg
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution

More Information

UniProt
P01579 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0050796 regulation of insulin secretion
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032735 positive regulation of interleukin-12 production
GO:0032747 positive regulation of interleukin-23 production
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042508 tyrosine phosphorylation of Stat1 protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051712 positive regulation of killing of cells of other organism
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
Regulatory
For research purposes only

Applications of IFN Gamma

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 10ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate positive/negative controls.
ELISA
PHP050 may be used in ELISA applications with either AHP775 or AHP775B.
Western Blotting
PHP050 may be used under reducing or non-reducing western blotting conditions with either AHP775 or AHP775B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Interferon Gamma AHP775 0.1 mg E FN P* WB
Rabbit anti Human Interferon Gamma:Biotin AHP775B 50 µg E WB

Application Based External Images

Functional Assays

Product Specific References

References for IFN Gamma

  1. Grisar, J. et al. (2001) Phenotypic characteristics of human monocytes undergoing transendothelial migration.
    Arthritis Res. 3 (2): 127-32.
  2. Fros, J.J. et al. (2010) Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling.
    J Virol. 84 (20): 10877-87.
  3. Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
    Invest Ophthalmol Vis Sci. 52: 5278-86.
  4. Wienken, C.J. et al. (2010) Protein-binding assays in biological liquids using microscale thermophoresis.
    Nat Commun. 1: 100.
  5. Bakmiwewa, S.M. et al. (2016) Synergistic induction of CXCL10 by interferon-gamma and lymphotoxin-alpha in astrocytes: Possible role in cerebral malaria.
    Cytokine. 78: 79-86.