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IL-4

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Recombinant Bovine Interleukin-4

Product Type
Recombinant Protein
Specificity
IL-4

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Product Code Applications Pack Size List Price Your Price Qty
PBP006
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN 20 µg loader
List Price Your Price
loader

Interleukin-4 (IL-4) is produced by a range of bovine cells, including T and B lymphocytes, monocytes and macrophages, and plays an important role in the immune response.

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Further dilutions should be made in a buffer containing carrier protein.
Preparation
Recombinant protein expressed in Pichia pastoris
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Approx. Protein Concentrations
Total protein concentration 0.04 mg/ml after reconstitution
Protein Molecular Weight
12.6 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PBP006 has been used as a standard in a sandwich ELISA assay with MCA2371 as the capture antibody and MCA2372B for detection.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine Interleukin-4 MCA2371 E 0.5 mg loader
List Price Your Price
loader
Description Mouse anti Bovine Interleukin-4
Mouse anti Bovine Interleukin-4:Biotin MCA2372B E 0.25 mg loader
List Price Your Price
loader
Description Mouse anti Bovine Interleukin-4:Biotin

References for IL-4

  1. Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
    Vet Res. 43: 54.
  2. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-7.
  3. Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
    Vet Immunol Immunopathol. 237: 110268.
  4. Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
    PLoS One. 17 (12): e0278313.

Functional Assays

UniProt
P30367
Entrez Gene
IL4
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0042113 B cell activation
GO:0043066 negative regulation of apoptosis
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes

PBP006

148156 150935 151755 152494 152495 152496

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