IL-4
Recombinant Bovine Interleukin-4
- Product Type
- Recombinant Protein
- Specificity
- IL-4
Interleukin-4 (IL-4) is produced by a range of bovine cells, including T and B lymphocytes, monocytes and macrophages, and plays an important role in the immune response. |
- Target Species
- Bovine
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Further dilutions should be made in a buffer containing carrier protein. - Preparation
- Recombinant protein expressed in Pichia pastoris
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Purity
- >95% by SDS PAGE analysis
- Approx. Protein Concentrations
- Total protein concentration 0.04 mg/ml after reconstitution
- Protein Molecular Weight
- 12.6 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Functional Assays |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse anti Bovine Interleukin-4 | MCA2371 | E | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Bovine Interleukin-4 | ||||||
Mouse anti Bovine Interleukin-4:Biotin | MCA2372B | E | 0.25 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Bovine Interleukin-4:Biotin |
References for IL-4
-
Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 43: 54. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7. -
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
Vet Immunol Immunopathol. 237: 110268. -
Barone, L.J. et al. (2023) Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages.
Virulence. Article: 2283899. -
Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
PLoS One. 17 (12): e0278313.
- UniProt
- P30367
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0042113 B cell activation
- GO:0043066 negative regulation of apoptosis
- GO:0045348 positive regulation of MHC class II biosynthetic process
- View More GO Terms
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Bovine ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up