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IgG

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Bovine IgG (PBP002) detected using anti Bovine/Ovine IgG/A/M (MCA625GA) and Goat anti Mouse IgG (STAR117P) as the secondary antibody.

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Purified Bovine IgG

Product Type
Purified Protein
Specificity
IgG

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PBP002
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Purified Bovine IgG is a preparation of purified polyclonal bovine IgG prepared from normal bovine serum.
By immunoelectrophoresis, this product was shown to react with antiserum specific for bovine IgG and subclasses IgG1 and IgG2. No reactivity was observed with antiserum for bovine IgA or IgM.

Purified Bovine IgG (PBP002) contains <1% IgA and IgM as determined by ELISA.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified normal bovine IgG prepared by fractionation and DEAE chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG

  1. Al-Alo, K.Z.K. et al. (2018) Correlation between neonatal calf diarrhea and the level of maternally derived antibodies.
    Iran J Vet Res. 19 (1): 3-8.

RRID
AB_324784
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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