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IFN Gamma

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Published customer image:
Recombinant Bovine interferon γ (PBP007) used to stimulate bovine endothelial luteal cells in vitro prior to metabolite analysis by PCR and western blotting.
Image caption:
mRNA and protein expression for selected aa metabolites in non- and cytokine treated EnCL-1 cells. LTC4 synthase, LTA4 hydrolase, PGE2 synthase, PGF2α synthase and EDN-1 (A) mRNA, (B) protein expression of non-treated and cytokine (TNFα/IFNγ) stimulated EnCL-1 cells and (C) representive immunoblots of studies factors: 1 lane: Positive Control-bovine lung tissue, 2 lane-Control, 3 lane-TNFα/IFNγ. Arrows indicate statistical differences in the respective mRNA quantitative expression between groups of non-treated and cytokine treated cells (P<0.05), as determined by one-way ANOVA followed by t-student's multiple comparison test.

From: Korzekwa AJ, Bodek G, Bukowska J, Blitek A, Skarzynski DJ.
Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 2011 Feb 24;9:27.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Recombinant Bovine Interferon Gamma

Product Type
Recombinant Protein
Specificity
IFN Gamma

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Product Code Applications Pack Size List Price Your Price Qty
PBP007A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN 25 µg loader
List Price Your Price
loader

Recombinant Bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.

IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 100 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
16.9 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent has been used as a standard in a sandwich ELISA assay in conjunction with clone CC330 (MCA2112) and clone CC302 (MCA1783B)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine Interferon Gamma:Biotin MCA1783B E 0.25 mg loader
List Price Your Price
loader
Description Mouse anti Bovine Interferon Gamma:Biotin
Mouse anti Bovine Interferon Gamma MCA2112 E ES F * 0.5 mg loader
List Price Your Price
loader
Description Mouse anti Bovine Interferon Gamma

References for IFN Gamma

  1. Floss, M.M. et al. (2010) Expression and Immunogenicity of the Mycobacterial Ag85B/ESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy.
    J Biomed Biotechnol. 2010: 274346.
  2. Korzekwa, A.J. et al. (2011) Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
    Reprod Biol Endocrinol. 9: 27.
  3. Pujol, J. et al. (2015) Generation of a soluble recombinant trimeric form of bovine CD40L and its potential use as a vaccine adjuvant in cows.
    Vet Immunol Immunopathol. 168 (1-2): 1-13.
  4. Soria, I. et al. (2018) Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle.
    J Immunol Res. 2018: 3497401.
  5. Hecker, Y.P. et al. (2019) Immune response to Neospora caninum live tachyzoites in prepubertal female calves.
    Parasitol Res. 118 (10): 2945-55.
  6. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-907.
  7. Fiorani, F. et al. (2019) Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum. tachyzoite antigen in previously exposed cattle.
    Vet Immunol Immunopathol. 207: 31-5.
  8. Soria, I. et al. (2017) Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle.
    PLoS One. 12 (9): e0185184.
    9. View The Latest Product References
  9. Kornuta, C.A. et al. (2022) TLR activation, immune response and viral protection elicited in cattle by a commercial vaccine against Bovine Herpesvirus-1.
    Virology. 566: 98-105.
  10. Bouroutzika, E. et al. (2023) Melatonin Administration to Pregnant Ewes for Coccidiosis Control in Their Offspring.
    Animals (Basel). 13 (14): 2381.
  11. Hashem, Z. et al. (2023) Development, Preparation and Evaluation of a Murine Monoclonal AntibodiesBased Interferon-Gamma Enzyme-Linked Immunosorbent Assay (ELISA) for Rapid and Accurate Diagnosis of Bovine Tuberculosis
    Int J Vet Sci. 12 (4): 599-606.
  12. Lybeck, K. et al. (2024) Selection of vaccine-candidate peptides from Mycobacterium avium subsp. paratuberculosis by in silico prediction, in vitro T-cell line proliferation, and in vivo immunogenicity.
    Front Immunol. 15: 1297955.

Synonyms
Interferon Gamma
UniProt
P07353
Entrez Gene
IFNG
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response

PBP007A

150721 159882 163730 167142

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