IFN Gamma

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Recombinant Bovine Interferon Gamma

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP007A E FN 25 µg
Recombinant Bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.

IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.

Product Details

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 100 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
16.9 kDa

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life
12 months from date of despatch

More Information

UniProt
P07353 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IFN Gamma

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent has been used as a standard in a sandwich ELISA assay in conjunction with clone CC330 (MCA2112) and clone CC302 (MCA1783B)

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine Interferon Gamma:Biotin MCA1783B 0.25 mg E
Mouse anti Bovine Interferon Gamma MCA2112 0.5 mg E ES F*

Product Specific References

References for IFN Gamma

  1. Floss, M.M. et al. (2010) Expression and Immunogenicity of the Mycobacterial Ag85B/ESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy.
    J Biomed Biotechnol. 2010: 274346.
  2. Korzekwa, A.J. et al. (2011) Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
    Reprod Biol Endocrinol. 9: 27.
  3. Pujol, J. et al. (2015) Generation of a soluble recombinant trimeric form of bovine CD40L and its potential use as a vaccine adjuvant in cows.
    Vet Immunol Immunopathol. 168 (1-2): 1-13.
  4. Soria, I. et al. (2018) Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle.
    J Immunol Res. 2018: 3497401.