IFN Gamma
Recombinant Bovine interferon γ (PBP007) used to stimulate bovine endothelial luteal cells in vitro prior to metabolite analysis by PCR and western blotting.
Image caption:
mRNA and protein expression for selected aa metabolites in non- and cytokine treated EnCL-1 cells. LTC4 synthase, LTA4 hydrolase, PGE2 synthase, PGF2α synthase and EDN-1 (A) mRNA, (B) protein expression of non-treated and cytokine (TNFα/IFNγ) stimulated EnCL-1 cells and (C) representive immunoblots of studies factors: 1 lane: Positive Control-bovine lung tissue, 2 lane-Control, 3 lane-TNFα/IFNγ. Arrows indicate statistical differences in the respective mRNA quantitative expression between groups of non-treated and cytokine treated cells (P<0.05), as determined by one-way ANOVA followed by t-student's multiple comparison test.
From: Korzekwa AJ, Bodek G, Bukowska J, Blitek A, Skarzynski DJ.
Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 2011 Feb 24;9:27.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Recombinant Bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.
Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.
IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.
IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
Product Details
- Target Species
- Bovine
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 100 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.9 kDa
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07353
- Entrez Gene
- IFNG
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IFN Gamma
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine Interferon Gamma:Biotin | MCA1783B | E | 0.25 mg |
|
|
| Mouse anti Bovine Interferon Gamma | MCA2112 | E ES F* | 0.5 mg |
|
Product Specific References
References for IFN Gamma
-
Floss, M.M. et al. (2010) Expression and Immunogenicity of the Mycobacterial Ag85B/ESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy.
J Biomed Biotechnol. 2010: 274346. -
Korzekwa, A.J. et al. (2011) Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 9: 27. -
Pujol, J. et al. (2015) Generation of a soluble recombinant trimeric form of bovine CD40L and its potential use as a vaccine adjuvant in cows.
Vet Immunol Immunopathol. 168 (1-2): 1-13. -
Soria, I. et al. (2018) Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle.
J Immunol Res. 2018: 3497401. -
Hecker, Y.P. et al. (2019) Immune response to Neospora caninum live tachyzoites in prepubertal female calves.
Parasitol Res. 118 (10): 2945-55. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-907. -
Fiorani, F. et al. (2019) Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum. tachyzoite antigen in previously exposed cattle.
Vet Immunol Immunopathol. 207: 31-5.
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