IFN Gamma
Recombinant Bovine interferon &gamma used to stimulate bovine endothelial luteal cells in vitro prior to metabolite analysis by PCR and western blotting.
Image caption:
mRNA and protein expression for selected aa metabolites in non- and cytokine treated EnCL-1 cells. LTC4 synthase, LTA4 hydrolase, PGE2 synthase, PGF2α synthase and EDN-1 (A) mRNA, (B) protein expression of non-treated and cytokine (TNFα/IFNγ) stimulated EnCL-1 cells and (C) representive immunoblots of studies factors: 1 lane: Positive Control-bovine lung tissue, 2 lane-Control, 3 lane-TNFα/IFNγ. Arrows indicate statistical differences in the respective mRNA quantitative expression between groups of non-treated and cytokine treated cells (P<0.05), as determined by one-way ANOVA followed by t-student's multiple comparison test.
From: Korzekwa AJ, Bodek G, Bukowska J, Blitek A, Skarzynski DJ.
Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 2011 Feb 24;9:27.
Recombinant Bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.
Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.
IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.
IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
Product Details
- Target Species
- Bovine
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 100 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.9 kDa
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P07353 Related reagents
- Entrez Gene
- IFNG Related reagents
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IFN Gamma
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine Interferon Gamma:Biotin | MCA1783B | 0.25 mg | E | ||
| Mouse anti Bovine Interferon Gamma | MCA2112 | 0.5 mg | E ES F* |
Product Specific References
References for IFN Gamma
-
Floss, M.M. et al. (2010) Expression and Immunogenicity of the Mycobacterial Ag85B/ESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy.
J Biomed Biotechnol. 2010: 274346. -
Korzekwa, A.J. et al. (2011) Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 9: 27. -
Pujol, J. et al. (2015) Generation of a soluble recombinant trimeric form of bovine CD40L and its potential use as a vaccine adjuvant in cows.
Vet Immunol Immunopathol. 168 (1-2): 1-13.
