Tremelimumab antibody | AbD64580
Human anti Tremelimumab:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD64580
- Isotype
- Fab antibody
- Specificity
- Tremelimumab
Antibody Quality and Performance Guarantee
TZA0127P
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Tremelimumab Antibody, clone AbD64580ad (TZA0127) at a concentration of 2 μg/mL in PBST followed by an HRP labelled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with Tremelimumab, human CTLA-4, 10% NHS, human IgG1 kappa control and human IgG1 lambda control at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Tremelimumab Antibody, clone AbD64580ad (TZA0127) titrated to the given concentrations in PBST followed by an HRP conjugated Anti-DYKDDDDK tag Antibody in HISPEC diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for tremelimumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Tremelimumab Antibody, clone AbD64576ad (TZA0126) at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of tremelimumab. Detection was performed using HRP conjugated Human Anti-Tremelimumab Antibody, clone AbD64580pap (TZA127P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Tremelimumab Antibody, clone AbD64580 is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the humanized monoclonal antibody drug tremelimumab. It does not recognize the drug target CTLA-4, nor tremelimumab in complex with CTLA-4. This antibody can be used to measure free tremelimumab levels in serum from patients. TZA0127 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0127. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA0127P. A pair of anti-tremelimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD64580 is recommended as a detection antibody, paired with Human Anti-Tremelimumab Antibody, clone AbD64576ad (TZA0126) as the capture antibody. Tremelimumab (trade name Imjudo) is a fully humanized monoclonal IgG2/kappa antibody with allotype IGHG2*01. It binds to cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) to enhance T cell-mediated killing of tumours and reduces tumour growth. Tremelimumab in combination with durvalumab and platinum-based chemo-therapy is indicated for the first-line treatment of adults with metastatic non-small cell lung cancer (NSCLC) with no sensitising EGFR mutations or ALK positive mutations. View a summary of all anti-tremelimumab antibodies |
- Product Form
- A bivalent human recombinant Fab (kappa light chain) generated by coupling of the monovalent SpyTagged Fab TZA0127 with a HRP conjugated BiSpyCatcher version 2 (TZC002P). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Tremelimumab
- Affinity
- The monovalent intrinsic affinity of AbD64580pap was measured as KD = 0.2 nM by real time, label free molecular interaction analysis on immobilized tremelimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Imjudo is a trademark of Pfizer and now investigated by MedImmune (AstraZeneca). - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD64580pap may be used as the detection antibody together with AbD64576ad (TZA0126) as the capture antibody for tremelimumab in a PK bridging ELISA.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Tremelimumab | TZA0126 | E | 0.1 mg |
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| Description | Human anti Tremelimumab | ||||||
| Human anti Tremelimumab | TZA0127 | E | 0.1 mg |
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| Description | Human anti Tremelimumab | ||||||
| Human anti Tremelimumab (Drug/Target Complex) | TZA0128 | E | 0.1 mg |
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| Description | Human anti Tremelimumab (Drug/Target Complex) | ||||||
| Human anti Tremelimumab (Drug/Target Complex):HRP | TZA0128P | E | 0.1 mg |
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| Description | Human anti Tremelimumab (Drug/Target Complex):HRP | ||||||
- Synonyms
- Imjudo
TZA0127P
172654
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Tremelimumab specificity
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