Ixekizumab antibody | AbD60570ad
Human anti Ixekizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD60570ad
- Isotype
- Fab antibody
- Specificity
- Ixekizumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ixekizumab Antibody, clone AbD60570ad (TZA0105) at a concentration of 2 μg/ml followed by HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with ixekizumab, nivolumab, IgG4 isotype control, human IgG4 kappa control and human IgG4 lambda control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ixekizumab Antibody, clone AbD60570ad (TZA0105) titrated to the given concentrations in PBST followed by an HRP conjugated Anti-DYKDDDDK tag Antibody in HISPEC diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for ixekizumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Ixekizumab Antibody, clone AbD60570ad (TZA0105) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of ixekizumab. Detection was performed using HRP conjugated Human Anti-Ixekizumab Antibody, clone AbD60557pap (TZA0106P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant human IL-17A at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of ixekizumab (0.15 µg/ml) plus increasing concentrations of Human Anti-Ixekizumab Antibody, clone AbD60570ad (TZA0105) was added. Free ixekizumab, still capable of binding to the human IL-17A coated plate was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. The non-inhibitory Human Anti-Ixekizumab Antibody, clone AbD60554ad (TZA0107) was used as a negative control. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Ixekizumab Antibody, clone AbD60570ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug ixekizumab, and its biosimilar products. It does not recognize the drug target, human Interleukin 17 (IL-17A), nor ixekizumab in complex with IL-17A. This antibody can be used to measure free ixekizumab levels in serum from patients. A pair of anti-ixekizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD60570ad is recommended as a capture antibody, paired with Human Anti-Ixekizumab Antibody, clone AbD60557 (TZA0106) as the detection antibody. TZA0105 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. Ixekizumab (trade name Taltz) is a humanized monoclonal antibody of the IgG4/kappa isotype that binds to interleukin 17 (IL-17A), a pro-inflammatory cytokine, and blocks its action. Blocking its effect is beneficial for use in inflammatory conditions. Ixekizumab is indicated for the treatment of moderate-to-severe plaque psoriasis and as a second-line therapy for active psoriatic arthritis. View a summary of all anti-ixekizumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library and expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Ixekizumab
- Affinity
- The monovalent intrinsic affinity of Human anti-Ixekizumab Antibody, clone AbD60570ad was measured as KD = 0.2 nM by real time, label-free molecular interaction analysis on immobilized ixekizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Taltz is a registered trademark of Eli Lily. - Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Clone AbD60570ad can be used in indirect ELISA to detect ixekizumab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for risankizumab, together with TZA0106 or TZA0106P as the detection reagent.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Ixekizumab | HCA432 | E | 0.1 mg |
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| Description | Human anti Ixekizumab | ||||||
| Human anti Ixekizumab | HCA433 | E | 0.1 mg |
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| Description | Human anti Ixekizumab | ||||||
| Human anti Ixekizumab | HCA434 | E | 0.1 mg |
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| Description | Human anti Ixekizumab | ||||||
| Human anti Ixekizumab | HCA435 | E | 0.1 mg |
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| Description | Human anti Ixekizumab | ||||||
| Human anti Ixekizumab | TZA0106 | E | 0.1 mg |
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| Description | Human anti Ixekizumab | ||||||
| Human anti Ixekizumab:HRP | TZA0106P | E | 0.1 mg |
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| Description | Human anti Ixekizumab:HRP | ||||||
| Human anti Ixekizumab | TZA0107 | E | 0.1 mg |
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| Description | Human anti Ixekizumab | ||||||
| Human anti Ixekizumab:HRP | TZA0107P | E | 0.1 mg |
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| Description | Human anti Ixekizumab:HRP | ||||||
- Synonyms
- Taltz
TZA0105
171365
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Ixekizumab specificity
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