SARS-CoV-2 Spike Protein S2 antibody

Rabbit anti SARS-CoV-2 Spike Protein S2

Product Type
Polyclonal Antibody
Isotype
IgG
Specificity
SARS-CoV-2 Spike Protein S2

Product Code Applications Pack Size List Price Your Price Qty
AHP3016
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Rabbit anti SARS-CoV-2 spike protein S2 antibody recognizes spike glycoprotein, also known as S glycoprotein, peplomer protein and E2.

Human coronaviruses are pathogens that can cause severe respiratory tract infections. SARS-CoV-2 is a novel human coronavirus characterized in 2020, and causes "coronavirus disease 2019" or "COVID-19" (Xu et al. 2020). The spike glycoproteins of coronaviruses are incorporated into the viral envelope and promote entry into cells (Walls et al. 2020). The spike glycoprotein of SARS-CoV-2 is comprised of two subunits called S1 and S2. The S1 protein binds to a receptor on human cells, and the transmembrane unit S2 helps fuse the viral membrane with a cellular membrane (Hoffmann et al. 2020). Specifically, both SARS-CoV and SARS-CoV-2 spike proteins contain a receptor-binding domain (RBD) that recognize the same receptor, ACE2, on human cells (Shang et al. 2020). This antibody recognizes the spike S2 protein of SARS-CoV-2.

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Peptide corresponding to 16 amino acids near the center terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein.

The immunogen is located within 900-950 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 10 ug/ml
Western Blotting 1 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P0DTC2
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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