Human Immunodeficiency Virus 1 p24 antibody
Goat anti HIV p24 antibody (4999-9007) used to analyse p24 protein expression by western blotting.
Image caption:
Virus extracts corresponding to the indicated p24 amount were resolved on PAGE-SDS gels, and immunoblotted with specifc antibodies for their content in p24 and HA-Vpr.
From: Eldin P, Péron S, Galashevskaya A, et al.
Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination.
J Transl Med. 2020;18(1):310.
doi:10.1186/s12967-020-02478-7
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Human immunodeficiency virus 1 p24 antibody recognizes the HIV1 viral capsid p24 protein encoded by the gag gene along with p6, p7 and p17 which provide structural elements of the virus.
Goat anti Human immunodeficiency virus 1 p24 antibody does not cross-react with bovine sera, human sera or human T or B cells.
Goat anti Human immunodeficiency virus 1 p24 antibody does not cross-react with bovine sera, human sera or human T or B cells.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.002% Thiomersal 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.1% Sodium azide (NaN3).
- Immunogen
- Purified native p24, from strain IIIB.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- 4.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Human Immunodeficiency Virus 1 p24 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/20 | 1/200 | |
| Immunofluorescence | |||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for Human Immunodeficiency Virus 1 p24 antibody
-
Mazzolini, J. et al. (2010) Inhibition of phagocytosis in HIV-1-infected macrophages relies on Nef-dependent
alteration of focal delivery of recycling compartments.
Blood 115: 4226-36. -
Fenard, D. et al. (2009) Uracil DNA Glycosylase 2 negatively regulates HIV-1 LTR transcription.
Nucleic Acids Res. 37: 6008-18. -
Eldin, P. et al. (2014) Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA.
Nucleic Acids Res. 42 (3): 1698-710. -
Singh, K.K. et al. (2011) Expression of mannose binding lectin in HIV-1-infected brain: implications for HIV-related neuronal damage and neuroAIDS.
Neurobehav HIV Med. 3: 41-52. -
Dumas, A. et al. (2015) The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking.
J Cell Biol. 211 (2): 359-72. -
Eldin, P. et al. (2020) Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination.
J Transl Med. 18 (1): 310.
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