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Human Immunodeficiency Virus 1 p24 antibody

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Anti Human Immunodeficiency Virus 1 p24 Antibody thumbnail image 1
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Published customer image:
Goat anti HIV p24 antibody (4999-9007) used to analyse p24 protein expression by western blotting.
Image caption:
Virus extracts corresponding to the indicated p24 amount were resolved on PAGE-SDS gels, and immunoblotted with specifc antibodies for their content in p24 and HA-Vpr.

From: Eldin P, Péron S, Galashevskaya A, et al.
Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination.
J Transl Med. 2020;18(1):310.
doi:10.1186/s12967-020-02478-7
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Goat anti Human Immunodeficiency Virus 1 p24

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Human Immunodeficiency Virus 1 p24

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Product Code Applications Pack Size List Price Your Price Qty
4999-9007
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 1 ml loader
List Price Your Price
loader

Goat anti Human immunodeficiency virus 1 p24 antibody recognizes the HIV1 viral capsid p24 protein encoded by the gag gene along with p6, p7 and p17 which provide structural elements of the virus.

Goat anti Human immunodeficiency virus 1 p24 antibody does not cross-react with bovine sera, human sera or human T or B cells.

Target Species
Viral
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium azide (NaN3).
Immunogen
Purified native p24, from strain IIIB.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Human Immunodeficiency Virus 1 p24 antibody

  1. Fenard, D. et al. (2009) Uracil DNA Glycosylase 2 negatively regulates HIV-1 LTR transcription.
    Nucleic Acids Res. 37: 6008-18.
  2. Mazzolini, J. et al. (2010) Inhibition of phagocytosis in HIV-1-infected macrophages relies on Nef-dependent alteration of focal delivery of recycling compartments.
    Blood 115: 4226-36.
  3. Singh, K.K. et al. (2011) Expression of mannose binding lectin in HIV-1-infected brain: implications for HIV-related neuronal damage and neuroAIDS.
    Neurobehav HIV Med. 3: 41-52.
  4. Eldin, P. et al. (2014) Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA.
    Nucleic Acids Res. 42 (3): 1698-710.
  5. Dumas, A. et al. (2015) The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking.
    J Cell Biol. 211 (2): 359-72.
  6. Eldin, P. et al. (2020) Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination.
    J Transl Med. 18 (1): 310.

Immunofluorescence

Immunohistology - Frozen

Western Blotting

RRID
AB_620016

4999-9007

153763 161081

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