Human Immunodeficiency Virus 1 gp120 antibody
Goat anti Human Immunodeficiency Virus 1 gp120 antibody (AHP2204) used for evaluation of envelope protein fragments in HeLa cell lysates by western blotting.
Image caption:
FIP1C depletion and HIV-1 Env incorporation in HeLa cells. (A) The efficiency of depletion of Rab11a-FIPs was measured by real time PCR (top). Infectivity of released particles following depletion of each FIP family member is shown below. (B) Depletion of FIP1C was confirmed at the protein level by immunoblotting with a FIP1C-specific antiserum. Env levels in both cell lysates and virions harvested from the FIP-depleted HeLa cells were assayed by immunoblotting. (C) Restoration of Env incorporation following shRNA-mediated depletion. Two constructs were used in this experiment: FIP1C cDNA is an shRNA sensitive GFP-tagged plasmid; while FIP1C* includes silent mutations in the shRNA target sequence rendering it shRNA-resistant. 2 ug and 4 ug of each FIP1C construct were used in the repletion assay as indicated at the top of the blot. (D) Infectivity of viral particles from the experiment shown in panel D as evaluated using TZM-bl indicator cells. Lanes are numbered and correspond to the blot above. Statistical comparisons utilized the unpaired t-test, * = p<.05; ** = p<.01.
From: Qi M, Williams JA, Chu H, Chen X, Wang J-J, Ding L, et al. (2013) Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting and Particle Incorporation of the HIV-1 Envelope Glycoprotein Complex.
PLoS Pathog 9(4): e1003278.
Goat anti Human Immunodeficiency Virus 1 gp120 antibody (AHP2204) used for evaluation of envelope protein fragments in HeLa cell lysates by western blotting.
Image caption:
ab14 enhancement of HIV-1 Env incorporation requires FIP1C and Rab14 depletion does not alter incorporation of MLV or VSV-G Env. A) Titration of Rab14Q70L in HeLa cells with normal FIP1C levels (Scr shRNA lanes) or in cells depleted of FIP1C (FIP1C shRNA lanes). Note that gp120 and gp160 blots were probed with goat anti-gp120/gp160 antisera, while gp41 was probed with murine monoclonal anti-gp41 antibody. B) Hela cells were transfected with NL4-3 or with NL4-3deltaEnv and expression constructs pHCMV-G (for VSV-G) or pCL-Ampho (MLV Env). Depletion of Rab14 was performed using shRNA in the indicated lanes. Cellular and particle Env content was assessed by immunoblotting with specific antisera for HIV gp120/160, VSV-G, or amphotropic MLV Env.
From: Qi M, Williams JA, Chu H, Chen X, Wang J-J, Ding L, et al. (2013) Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting and Particle Incorporation of the HIV-1 Envelope Glycoprotein Complex.
PLoS Pathog 9(4): e1003278..
Goat anti Human Immunodeficiency Virus 1 gp120 antibody recognizes the human immunodeficiency virus envelope protein. The viral envelope protein gp160 is cleaved into fragments gp120 and gp41. gp120 is an exterior envelope glycoprotein on the surface of the virus particle that binds to CD4 and chemokine receptors on macrophages and T-lymphocytes. gp41 is non-covalently bound to gp120 and aids the virus in entering the cell.
Goat anti Human Immunodeficiency Virus 1 gp120 antibody reacts with gp120, gp160 and gp41 in western blot assays.
Goat anti Human Immunodeficiency Virus 1 gp120 antibody reacts with gp120, gp160 and gp41 in western blot assays.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant gp160 comprising the C terminus of gp120 and most of gp41.
- Purity
- >98%
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q70626 Related reagents
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005198 structural molecule activity
- GO:0019031 viral envelope
- GO:0019059 initiation of viral infection
- GO:0020002 host cell plasma membrane
- GO:0030260 entry into host cell
- GO:0030683 evasion by virus of host immune response
- GO:0044175 host cell endosome membrane
- GO:0055036 virion membrane
- Regulatory
- For research purposes only
Applications of Human Immunodeficiency Virus 1 gp120 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/10,000 | |
| Western Blotting | 1/500 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Product Specific References
References for Human Immunodeficiency Virus 1 gp120 antibody
-
Qi, M. et al. (2013) Rab11-FIP1C and Rab14 direct plasma membrane sorting and particle incorporation of the HIV-1 envelope glycoprotein complex.
PLoS Pathog. 9 (4): e1003278. -
Qi, M. et al. (2015) A tyrosine-based motif in the HIV-1 envelope glycoprotein tail mediates cell-type- and Rab11-FIP1C-dependent incorporation into virions.
Proc Natl Acad Sci U S A. 112 (24): 7575-80.
