Human Immunodeficiency Virus 1 gp120 antibody

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Goat anti Human Immunodeficiency Virus 1 gp120:Biotin

Goat anti Human Immunodeficiency Virus 1 gp120:HRP

Goat anti Human Immunodeficiency Virus 1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5000-0567 E WB 1 ml
5000-0587 C E WB 1 ml
5000-0557 C E WB 1 ml
Goat anti Human Immunodeficiency Virus 1 gp120 antibody recognizes gp120, an exterior envelope glycoprotein on the surface of the virus particle that acts as a receptor for CD4 and chemokine receptors on macrophages and T-lymphocytes.

It does not react with human T cells, B cells, normal bovine serum or normal human serum. In western blotting a predominant gp120 band is observed.

Product Details

Target Species
Viral
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.002%Thiomersal
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified native gp120 from strain IIIB.
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q70626 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005198 structural molecule activity
GO:0019031 viral envelope
GO:0019059 initiation of viral infection
GO:0020002 host cell plasma membrane
GO:0030260 entry into host cell
GO:0030683 evasion by virus of host immune response
GO:0044175 host cell endosome membrane
GO:0055036 virion membrane
Regulatory
For research purposes only

Applications of Human Immunodeficiency Virus 1 gp120 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000
ELISA 1/200 1/1000
Immunohistology - Frozen 1/20 1/200
Western Blotting 1/20 1/200
ELISA
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Human Immunodeficiency Virus 1 gp120 antibody

  1. Reynard, F. et al. (2004) HIV-1 acute infection env glycomutants designed from 3D model: effects on processing, antigenicity, and neutralization sensitivity.
    Virology. 324: 90-102.