Herpes simplex Virus 1/2 antibody
Goat anti Herpes simplex virus 1/2 antibody (4956-0104) used to demonstrate HSV infection of tonsil cells by immunofluorescence.
Image caption:
HIV tat/gp120-disrupted epithelial junctions facilitate HSV-1 cell-to-cell spread through junctional areas of polarized oral epithelial cells.
A. Polarized tonsil cells were treated with active or inactive tat/gp120 and HIV virions for 5 days. Disrupted cells were infected with HSV-1 at 0.01 PFU per cell from basolateral membranes of polarized cells. After 3 days, cells were fixed and immunostained using goat anti-HSV immune serum (green). Cell nuclei are stained in red. Yellow represents colocalization of HSV proteins and nuclei. B. (upper panel) Plaque numbers were counted from 3 independent filter inserts and data are presented as the average number of HSV-infected plaques per insert. (lower panel) Cell-to-cell spread of HSV-1 was quantitatively evaluated by counting HSV-infected cells in the plaques. Results are presented as the average number of HSV-infected cells per plaque. Error bars indicate SEM. C. Polarized cells were infected with HSV-1. After 4 h, antibodies to nectin-1 and gD were added separately and in combination. Cell medium was changed daily to add fresh antibodies. Cells were fixed and immunostained for HSV-1, and the plaque numbers (upper panel) and the number of HSV-1-positive cells in plaques were counted (lower panel). Error bars indicate SEM. *P<0.05, *P<0.01, **P<0.001, all compared with the control group.
From: Sufiawati I, Tugizov SM (2014)
HIV-Associated Disruption of Tight and Adherens Junctions of Oral Epithelial Cells Facilitates HSV-1 Infection and Spread.
PLoS ONE 9(2): e88803.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Herpes simplex virus antibody (4956-0104) used for the evaluation of HSV1 expression in infected Vero cell cultures by western blotting.
Image caption:
Cell lysates were analyzed by Western blotting (C) with anti-HSV-1 antibodies.
From: Di Sotto A, Di Giacomo S, Amatore D, Locatelli M, Vitalone A, Toniolo C, Rotino GL, Lo Scalzo R, Palamara AT, Marcocci ME, Nencioni L.
A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties andAnti-Herpes Simplex Virus Type 1 Activity In Vitro. Molecules. 2018 Aug 17;23(8). pii: E2066.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Herpes simplex Virus 1/2
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Herpes simplex Virus 1/2
Antibody Quality and Performance Guarantee
| Goat anti Herpes simplex Virus 1/2 antibody reacts with Herpes Simplex virus 1 and 2, recognizing infected cell proteins (ICPs) and late structural (virion) antigens. Goat anti Herpes simplex Virus 1/2 antibody does not react with HEp-2 cells. Herpes Simplex virus (HSV) is a double stranded DNA virus of which there are 2 types, HSV1 and HSV2. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From the trigeminal ganglion, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Human HSV type 1, Strain F
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for Herpes simplex Virus 1/2 antibody
-
Sufiawati, I. and Tugizov, S.M. (2014) HIV-associated disruption of tight and adherens junctions of oral epithelial cells facilitates HSV-1 infection and spread.
PLoS One. 9: e88803. -
Civitelli, L. et al. (2015) Herpes simplex virus type 1 infection in neurons leads to production and nuclear localization of APP intracellular domain (AICD): implications for Alzheimer's disease pathogenesis.
J Neurovirol. 21 (5): 480-90. -
Marcocci, M.E. et al. (2018) The Amphibian Antimicrobial Peptide Temporin B Inhibits In Vitro Herpes Simplex Virus 1 Infection.
Antimicrob Agents Chemother. 62 (5)Apr 26 [Epub ahead of print]. -
Di Sotto, A. et al. (2018) A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro.
Molecules. 23 (8)Aug 17 [Epub ahead of print].
- RRID
- AB_618826
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