IL-8 antibody

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Rabbit anti Sheep Interleukin-8

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP425 E 0.1 ml
Rabbit anti Sheep Interleukin-8 antibody recognises ovine interleukin-8, also known as IL-8, C-X-C motif chemokine 8 or Chemokine (C-X-C motif) ligand 8. Interleukin-8 is a 79 amino acid ~11kDa secreted cytokine chemotactic for neutrophils, basophils and T-cells but not monocytes.

No cross-reactivity is seen with ovine IL-1 beta, IL-6, MCP or TNF alpha.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Goat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to ovine IL-8 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant ovine IL-8.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P36925 Related reagents
Entrez Gene
IL8 Related reagents
GO Terms
GO:0005153 interleukin-8 receptor binding
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0008009 chemokine activity
GO:0030155 regulation of cell adhesion
GO:0030593 neutrophil chemotaxis
GO:0045091 regulation of retroviral genome replication
GO:0050930 induction of positive chemotaxis
Regulatory
For research purposes only

Applications of IL-8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-8, in combination with MCA1660 as capture antibody.

N.B. It is recommended that AHP425 and any detection antibody are diluted in 10% normal mouse serum prior to use in ELISA.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Application Based External Images

ELISA

Product Specific References

References for IL-8 antibody

  1. Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection.
    Infect Immun. 78: 3578-84.
  2. Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.
    Vet Pathol. 35 (2): 124-31.
  3. Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
    Physiol Genomics. 44 (7): 403-16.
  4. Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
    J Appl Physiol. 99: 2204-11.
  5. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  6. Stinson LF et al. (2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation in ex vivo human and ovine fetal membranes.
    Reproduction. 147 (3): 313-20.
  7. Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
    Am J Reprod Immunol. 73 (4): 372-82.
  8. Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
    Vet Res. 46: 9.