IL-8 antibody
Rabbit anti Sheep interleukin-8 antibody (AHP425) used in conjunction with Mouse anti Sheep interleukin-8 antibody, clone 8M6 (MCA1660 ) for the measurement of bovine CXCL8 in embryonal bovine lungs by ELISA.
Image caption:
Analysis of bovine TLR5 activity in bovine epithelial cells.
The bovine EBL cell line was stably transfected with a bTLR5 clone and CXCL8 secretion assayed following challenge with flagellin. (A) CXCL8 levels from EBLs with and without transfection of bTLR5. H7 flagellin at a range of concentrations was added to the cells and CXCL8 levels were measured by ELISA following overnight incubation. Transfection with bTLR5 resulted in significantly higher levels of CXCL8 being produced by the bTLR5+ cells with addition of 50-50,000 ng/mL of H7 flagellin (p < 0.001). The data shown are the means and 95% confidence intervals. (B) Analysis of CXCL8 production from EBL-TLR5 in response to addition of native H7 or an H7 flagellin preparation from which the majority of LPS has been removed. Medians and interquartile ranges are shown. (C) Secreted CXCL8 following addition of WT and mutated H7 flagellins to EBLs with transfected bTLR5. 50 ng/mL of WT-H7 and mutated flagellins were added to the EBLs transfected with bTLR5 and incubated overnight. CXCL8 was measured by ELISA. Addition of the R90T, R90A and L500A/I504A variants led to significantly lower levels of cytokine release (p <0.001) relative to WT-H7 stimulation (asterisks). R90T showed a significantly lower induction than R90A (p <0.001). Medians and interquartile ranges are shown. (D) Secreted CXCL8 following addition of WT H7 and the R90T flagellin mutant to EBL cells. A range of flagellin concentrations was incubated overnight with the cells and CXCL8 measured by ELISA. R90T flagellin demonstrated significantly reduced levels of CXCL8 activation at 50 ng/mL and 500 ng/mL (p <0.001), marked by asterisks. The data shown are the means and 95% confidence intervals. All CXCL8 data shown is from a minimum of three biological replicates.
From: Tahoun A, et al.
Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 2015 Feb 19;46:9.
Rabbit anti Sheep Interleukin-8 antibody (AHP425) used as a detection reagent in a sandwich ELISA to evaluate interleukin-8 levels in sheep neutrophil supernatants.
Image caption:
IL-8 production in PMN upon E. bovis exposure. Supernatants of PMN (1 × 106 per sample; n = 3) treated with TLR2 and TLR4 antibodies and exposed to live E. bovis sporozoites (1:1 ratio; 2 h) were assessed for the presence of IL-8 by ELISA analysis. TLR2-treated PMN exposed to sporozoites showed a significant increase in IL-8 production (* p <0.05) when compared to PMN in media. Likewise, a significant increase in IL-8 production (** p <0.01) was observed in the same experimental condition when compared to the respective control without exposure to E. bovis. Data are represented as the mean of 3 replicates ± SD and were analyzed using an unpaired Student’s t-test using GraphPad Prism V.8.4.3 (GraphPad Software Inc., San Diego, CA, USA). p-value notation; * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.
From: Muñoz-Caro T, Gibson AJ, Conejeros I, Werling D, Taubert A, Hermosilla C.
The Role of TLR2 and TLR4 in Recognition and Uptake of the Apicomplexan Parasite Eimeria bovis and Their Effects on NET Formation.
Pathogens. 2021 Jan 24;10(2):118.
doi: 10.3390/pathogens10020118.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Sheep Interleukin-8
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-8
Antibody Quality and Performance Guarantee
| Rabbit anti Sheep Interleukin-8 antibody recognises ovine interleukin-8, also known as IL-8, C-X-C motif chemokine 8 or Chemokine (C-X-C motif) ligand 8. Interleukin-8 is a 79 amino acid ~11kDa secreted cytokine chemotactic for neutrophils, basophils and T-cells but not monocytes. No cross-reactivity is seen with ovine IL-1 beta, IL-6, MCP or TNF alpha. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Goat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to ovine IL-8 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant ovine IL-8.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-8, in combination with MCA1660 as capture antibody.
N.B. It is recommended that AHP425 and any detection antibody are diluted in 10% normal mouse serum prior to use in ELISA.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
References for IL-8 antibody
-
Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.
Vet Pathol. 35 (2): 124-31. -
Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
J Appl Physiol. 99: 2204-11. -
Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection.
Infect Immun. 78: 3578-84. -
Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
Physiol Genomics. 44 (7): 403-16. -
Stinson LF et al. (2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation in ex vivo human and ovine fetal membranes.
Reproduction. 147 (3): 313-20. -
Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. 168 (1-2): 97-102. -
Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
Am J Reprod Immunol. 73 (4): 372-82. -
Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9.
View The Latest Product References
-
Santillo, A. et al. (2022) Feeding tannins to dairy cows in different seasons improves the oxidative status of blood plasma and the antioxidant capacity of cheese.
J Dairy Sci. 105 (11): 8609-20. -
Muñoz-Caro, T. et al. (2021) The Role of TLR2 and TLR4 in Recognition and Uptake of the Apicomplexan Parasite Eimeria bovis and Their Effects on NET Formation.
Pathogens. 10 (2): 118.
- Synonyms
- CXCL8
- RRID
- AB_322153
- UniProt
- P36925
- Entrez Gene
- IL8
- GO Terms
- GO:0005153 interleukin-8 receptor binding
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007050 cell cycle arrest
- GO:0008009 chemokine activity
- GO:0030155 regulation of cell adhesion
- GO:0030593 neutrophil chemotaxis
- GO:0045091 regulation of retroviral genome replication
- View More GO Terms
- GO:0050930 induction of positive chemotaxis
AHP425
150780
161206
162870
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-8 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Sheep ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up