IL-6 antibody

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Rabbit anti Sheep Interleukin-6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP424 E WB 0.1 ml
Rabbit anti Ovine interleukin-6 antibody recognizes ovine interleukin-6 (IL-6). No cross-reactivity is seen with ovine IL-1 beta, IL-8, MCP or TNF alpha.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid (diluted)
Antiserum Preparation
Antisera to ovine IL-6 were raised by repeated immunisation of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant IL-6.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P29455 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-6, in combination with MCA1659 as a capture antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for IL-6 antibody

  1. Stephens, S. et al. (2003) Differences in cytokine synthesis by the subpopulations of dendritic cells from afferent lymph.
    Immunology. 120: 48-57.
  2. Herath, S. et al. (2009) Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility.
    Reprod Biol Endocrinol. 7: 55.
  3. Wang, Z. et al. (2008) Acute hypercapnia improves indices of tissue oxygenation more than dobutamine in septic shock.
    Am J Respir Crit Care Med. 177: 178-83.
  4. Su, F. et al (2007) Beneficial effects of ethyl pyruvate in septic shock from peritonitis.
    Arch Surg.142: 166-71.
  5. Karisnan, K. et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
    PLoS One. 10 (4): e0124390.
  6. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
    Reprod Sci. 22 (11): 1409-20.
  7. Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
    PLoS One. 10 (5): e0124144.
  8. Herry, C.L. et al. (2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
    PLoS One. 11 (4): e0153515.