TNF Alpha antibody

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Rabbit anti Rat TNF Alpha:Biotin

Rabbit anti Rat TNF Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAR33B
Datasheet
E WB
MSDS
50 µg loader

AAR33
Datasheet
E FN P WB
MSDS
0.1 mg loader

Rabbit anti Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFα), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFα is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFα is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Preservative Stabilisers
None present
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant rat TNF alpha (PRP16).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P16599
Entrez Gene
Tnf
GO Terms
GO:0001666 response to hypoxia
GO:0001775 cell activation
GO:0002037 negative regulation of L-glutamate transport
GO:0003009 skeletal muscle contraction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0009612 response to mechanical stimulus
GO:0019722 calcium-mediated signaling
GO:0008285 negative regulation of cell proliferation
GO:0042493 response to drug
GO:0014823 response to activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043525 positive regulation of neuron apoptosis
GO:0045840 positive regulation of mitosis
GO:0046325 negative regulation of glucose import
GO:0050806 positive regulation of synaptic transmission
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15 0.30ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0 ug/ml
Functional Assays 0.07 0.1 ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AAR33 as the capture reagent and PRP16 as the standard.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AAR33B as the detection reagent and PRP16 as the standard.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for TNF Alpha antibody

  1. Halin, S. et al. (2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is downregulated by the prostate tumor microenvironment.
    Neoplasia. 12: 336-45.
  2. Khandelwal, P.J. et al. (2010) Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model.
    Mol Neurodegener. 5: 47.
  3. Sandoval, J. et al. (2010) Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.
    Cell Mol Life Sci. 67: 1687-97.
  4. Irwin, M.W. et al. (1999) Tissue expression and immunolocalization of tumor necrosis factor-alpha in postinfarction dysfunctional myocardium.
    Circulation. 99: 1492-8.
  5. El-Kharashi, O.A. et al. (2011) The Potential Pharmacological and Histological Benefits of Carvedilol on the Hippocampal Post- Stroke Seizures in Rats
    Life Science Journal, 8: 951-60
  6. Elgarf, A.S. et al. (2014) Lipopolysaccharide repeated challenge followed by chronic mild stress protocol introduces a combined model of depression in rats: reversibility by imipramine and pentoxifylline.
    Pharmacol Biochem Behav. 126: 152-62.
  7. Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
    Diabetes. 64 (5): 1780-5.
  8. Savard, A. et al. (2013) Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.
    J Neuroinflammation. 10: 110.
  9. Alizadeh, A. et al. (2017) Neuregulin-1 positively modulates glial response and improves neurological recovery following traumatic spinal cord injury.
    Glia.Apr 29. [Epub ahead of print]
  10. Zhang, Y. et al. (2015) Simvastatin inhibits ischemia/reperfusion injury-induced apoptosis of retinal cells via downregulation of the tumor necrosis factor-α/nuclear factor-κB pathway.
    Int J Mol Med. 36 (2): 399-405.
  11. Kartha, S. et al. (2016) Development of a Rat Model of Mechanically Induced Tunable Pain and Associated Temporomandibular Joint Responses.
    J Oral Maxillofac Surg. 74 (1): 54.e1-10.
  12. Li, J. et al. (2016) Propofol reduces liver dysfunction caused by tumor necrosis factor-α production in Kupffer cells.
    J Anesth. 30 (3): 420-6.
  13. Dyck, S. et al. (2018) Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
    J Neuroinflammation. 15 (1): 90.