TNF Alpha antibody
Rabbit anti Rat TNF alpha antibody used for evaluation of TNF-α expression in rat brain be western blotting
Image caption:
α-Synuclein-induced neuro-inflammation and parkin protection.
Western blot of TNF-α and iNOS in striatal extracts analyzed on 10% NuPAGE SDS gel (Invitrogen) and I). Densitometric analysis of immunoblots, Asterisk is significantly different to LacZ control or as indicated, ANOVA, with Neumann Keuls multiple comparison N = 4, P <<0.05.
From: Khandelwal PJ, Dumanis SB, Feng LR, Maguire-Zeiss K, Rebeck G, Lashuel HA, Moussa CE.
Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model.
Mol Neurodegener. 2010 Nov 4;5:47.
Rabbit anti Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFα), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFα is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFα is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None Present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant rat TNF alpha (PRP16).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P16599
- Entrez Gene
- Tnf
- GO Terms
- GO:0001666 response to hypoxia
- GO:0001775 cell activation
- GO:0002037 negative regulation of L-glutamate transport
- GO:0003009 skeletal muscle contraction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0009612 response to mechanical stimulus
- GO:0019722 calcium-mediated signaling
- GO:0008285 negative regulation of cell proliferation
- GO:0042493 response to drug
- GO:0014823 response to activity
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043525 positive regulation of neuron apoptosis
- GO:0045840 positive regulation of mitosis
- GO:0046325 negative regulation of glucose import
- GO:0050806 positive regulation of synaptic transmission
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.15 | 0.30ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5 | 2.0 ug/ml | |
| Functional Assays | 0.07 | 0.1 ug/ml | |
| Immunohistology - Paraffin | |||
| Western Blotting | 0.1 | 0.2 ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AAR33 as the capture reagent and PRP16 as the standard.
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AAR33B as the detection reagent and PRP16 as the standard.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for TNF Alpha antibody
-
Halin, S. et al. (2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is downregulated by the prostate tumor microenvironment.
Neoplasia. 12: 336-45. -
Khandelwal, P.J. et al. (2010) Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model.
Mol Neurodegener. 5: 47. -
Sandoval, J. et al. (2010) Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.
Cell Mol Life Sci. 67: 1687-97. -
Irwin, M.W. et al. (1999) Tissue expression and immunolocalization of tumor necrosis factor-alpha in postinfarction dysfunctional myocardium.
Circulation. 99: 1492-8. -
El-Kharashi, O.A. et al. (2011) The Potential Pharmacological and Histological Benefits of Carvedilol on the Hippocampal Post- Stroke Seizures in Rats
Life Science Journal, 8: 951-60 -
Elgarf, A.S. et al. (2014) Lipopolysaccharide repeated challenge followed by chronic mild stress protocol introduces a combined model of depression in rats: reversibility by imipramine and pentoxifylline.
Pharmacol Biochem Behav. 126: 152-62. -
Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
Diabetes. 64 (5): 1780-5. -
Savard, A. et al. (2013) Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.
J Neuroinflammation. 10: 110. -
Alizadeh, A. et al. (2017) Neuregulin-1 positively modulates glial response and improves neurological recovery following traumatic spinal cord injury.
Glia.Apr 29. [Epub ahead of print] -
Zhang, Y. et al. (2015) Simvastatin inhibits ischemia/reperfusion injury-induced apoptosis of retinal cells via downregulation of the tumor necrosis factor-α/nuclear factor-κB pathway.
Int J Mol Med. 36 (2): 399-405. -
Kartha, S. et al. (2016) Development of a Rat Model of Mechanically Induced Tunable Pain and Associated Temporomandibular Joint Responses.
J Oral Maxillofac Surg. 74 (1): 54.e1-10. -
Li, J. et al. (2016) Propofol reduces liver dysfunction caused by tumor necrosis factor-α production in Kupffer cells.
J Anesth. 30 (3): 420-6. -
Dyck, S. et al. (2018) Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
J Neuroinflammation. 15 (1): 90. -
Li, J. et al. (2015) Targeting NF-κΒ and TNF-α Activation by Electroacupuncture to Suppress Collagen-induced Rheumatoid Arthritis in Model Rats.
Altern Ther Health Med. 21 (4): 26-34. -
Noguchi, J. et al. (2020) Sperm immunization and rat spermatogenesis: Dysfunctional blood‐testis barrier and perturbed Sertoli cell cytoskeleton
Andrology. 9 (2): 744-57.
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