Goat anti Rat IL-6 antibody recognizes rat interleukin 6.
IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide.
- Antiserum Preparation
- Antisera to rat IL-6 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant Rat IL-6 (PRP21).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
interleukin-6 receptor binding
cell redox homeostasis
negative regulation of protein kinase activity
response to mechanical stimulus
growth factor activity
negative regulation of cell proliferation
regulation of cell shape
response to drug
response to heat
response to cold
response to antibiotic
response to caffeine
response to nutrient levels
response to lipopolysaccharide
response to insulin stimulus
positive regulation of protein import into nucleus, translocation
response to cytokine stimulus
negative regulation of caspase activity
response to amino acid stimulus
regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of nitric oxide biosynthetic process
positive regulation of neuron differentiation
negative regulation of gluconeogenesis
positive regulation of DNA replication
positive regulation of JAK-STAT cascade
negative regulation of muscle organ development
negative regulation of cytokine secretion
response to calcium ion
response to electrical stimulus
response to protein stimulus
positive regulation of transmission of nerve impulse
positive regulation of ERK1 and ERK2 cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-6 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
- Anti rat IL-6: purified (AAR35) may be used in an indirect ELISA with recombinant rat IL-6 (PRP21) as the standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra