IgG antibody

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Goat ANTI-RABBIT IgG StarBright™ Blue 700

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
12004162
Datasheet
WB
SDS
80 µl loader

12004161
Datasheet
WB
SDS
400 µl loader

StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.

StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Goat Anti-Rabbit IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, mouse and rat.

Product Details

Target Species
Rabbit
Product Form
Purified IgG conjugated to StarBright Blue 700 - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
Antiserum Preparation
Antiserum to Rabbit IgG was raised by repeated immunisation of Goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin

Storage Information

Storage
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/2,500 1/5,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004163 WB 200 µl loader
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004164 WB 40 µl loader
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004165 WB 200 µl loader
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004166 WB 40 µl loader
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004167 WB 200 µl loader
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004168 WB 40 µl loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004158 WB 400 µl loader
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004159 WB 80 µl loader