IgG antibody
* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP)
Western botting analysis of human adipocyte lysates probed with rabbit primary antibodies followed by StarBright Blue 520 conjugated anti Rabbit IgG antibody (1200586).
Image caption:
Rosmarinic acid reduced C/EBPα, PPARγ and adiponectin protein production in human SGBS adipocytes. Western blot performed to examine the protein level of C/EBPα, PPARγ, and adiponectin.
From: Vasileva LV, Savova MS, Tews D, Wabitsch M, Georgiev MI.
Rosmarinic acid attenuates obesity and obesity-related inflammation in human adipocytes.
Food Chem Toxicol. 2021 Mar;149:112002.
doi: 10.1016/j.fct.2021.112002.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
StarBright Blue 700 conjugated Goat anti Rabbit IgG antibody (12004162) used to detect bount rabbit antibodies in western blotting.
Image caption:
The relative amount of proteins: RAGE (A), CML (B), HMGB1 (C), s100b (D) and the sum of 4HNE (E) in the lumbar spinal cord harvested from SOD1 transgenic mice with congenital ALS at 90, 120, and terminal time points. Data are presented as means ± S.E.M. Statistical differences in relative amount of protein respectively * 0.01 ≤p ≤ 0.05; Abbreviation: ctr—control group- 90 time point, n = 4; 90 n = 3, 120 n = 3, T n = 4—correspond to 90, 120 and terminal endpoints (experimental group).
From: Nowicka N, Szymańska K, Juranek J, Zglejc-Waszak K, Korytko A, Załęcki M, Chmielewska-Krzesińska M, Wąsowicz K, Wojtkiewicz J.
The Involvement of RAGE and Its Ligands during Progression of ALS in SOD1 G93A Transgenic Mice.
Int J Mol Sci. 2022 Feb 16;23(4):2184.
doi: 10.3390/ijms23042184.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Starbright 700 conjugated Goat anti Rabbit antibody(12004162) used to detect bound rabbit antibodies in wwestern blotting.
Image caption:
miR-302d suppresses TGFB-induced production of FN1.
(A) ICC staining and (B) representative Western blot of FN1 in transfected hRPE cells after 3 d incubation of TGFB1. The scale bar represents 50 μm. (C) The relative expression of FN1 normalized to total proteins (S1 Raw images). The values were further normalized to the "wo TGFB1" group. One-way ANOVA with Tukey’s multiple comparisons tests (n = 3) were performed. The error bars represent SD. ns, non-significant, *p < 0.05.
From: Hu X, Binter M, Hufendiek K, Tode J, Framme C, Fuchs H (2022)
MiR-302d inhibits TGFB-induced EMT and promotes MET in primary human RPE cells.
PLoS ONE 17(11): e0278158.
doi: 10.1371/journal.pone.0278158
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
StarBright Blue 700 conjugated Goatanti Rabbit IgG antibody (12004161) used to label bound rabbit antibodies in western blotting.
Image caption:
Evaluation of the effects of pesticides using the autophagy activity markers as endpoints. Representative band images of western blotting analyses of autophagosome markers LC3 (A), autophagy substrate marker p62 and internal control GAPDH (B) are shown. The ratios of LC3-II/LC3-I and p62/GAPDH were calculated by band intensities. Cells were exposed to each pesticide (100 μM) or a positive control chloroquine (30 µM) for 24 h. All data are shown as the mean ± SD. * p <0.05, * * p < 0.01 vs. the control group (one-way ANOVA followed by Dunnett’s post-hoc test).
From: Hirano T, Ikenaka Y, Nomiyama K, Honda M, Suzuki N, Hoshi N, Tabuchi Y.
An adverse outcome pathway-based approach to assess the neurotoxicity by combined exposure to current-use pesticides.
Toxicology. 2023 Dec;500:153687.
doi: 10.1016/j.tox.2023.153687.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
StarBright Blue 700 conjugated Goat anti Rat IgG antibody (12004162) used to detect bound Rabbit anti TPM-1 antibodies in western blotting.
Image caption:
Stability of TPM2-X16 and TPM1-X1 to increasing temperatures and to acidic and basic conditions. SDS-PAGE of TPM2-X16 (a) and TPM1-X1 (e) after incubation at increasing temperatures. SDS-PAGE of TPM2-X16 (b) and TPM1-X1 (f) after incubation at different pH.
From: Delfino, D. et al.
Allergenicity of tropomyosin variants identified in the edible insect Hermetia illucens (black soldier fly).
Food Chem 437(1) 137849.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
StarBright™ Blue 700 conjugated Goat anti Rabbit IgG antibody(12004162) used as a secondary antibody for the determination of protein abundance in cell line lysates by western blotting.
Image caption:
Protein abundance in SW48 cell line lysate with treatment. Western blot of the proteins MMUT, MTR and MAT2A compared to the loading control β actin (A). Quantified protein abundance relative to β-actin for MMUT (B), MTR(C), and MAT2A (D). CTL: Control, Cbl: cobalamin, HCY: homocystine
From: Garg, S.; Miousse, I.R.
Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line.
Nutrients 2024, 16, 997.
doi: 10.3390/nu16070997
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat ANTI-RABBIT IgG StarBright™ Blue 700
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores. StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot. Features and Benefits Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm) Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies Goat Anti-Rabbit IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, mouse and rat. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
- Antiserum Preparation
- Antiserum to Rabbit IgG was raised by repeated immunisation of Goats with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Blue 700 470 700 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Western Blotting |  |
1/2,500 | 1/5,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing. - Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
-
Li, T. et al. (2021) RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.
Nat Commun. 12 (1): 1055. -
Vasileva, L.V. et al. (2021) Rosmarinic acid attenuates obesity and obesity-related inflammation in human adipocytes.
Food Chem Toxicol. 149: 112002. -
Dunbar, K. et al. (2021) IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.
Life Sci Alliance. 4 (2)Dec 23 [Epub ahead of print]. -
Dunbar, K. et al. (2021) FAM83F regulates canonical Wnt signalling through an interaction with CK1&alpha.
Life Sci Alliance. 4 (2)Dec 24 [Epub ahead of print]. -
Holoch, D. et al. (2021) A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals.
Nat Genet. 53 (12): 1686-1697. -
Goad, D.W. et al. (2021) Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
Molecular Therapy - Oncolytics. Dec 01 [Epub ahead of print]. -
Reisman, B.J. et al. (2021) Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase.
Nat Chem Biol. Dec 02 [Epub ahead of print]. -
Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance. 5 (3)Dec 21 [Epub ahead of print].
View The Latest Product References
-
Meissner, M.E. et al. (2022) Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.
J Mol Biol. 434 (2): 167355. -
Nowicka, N. et al. (2022) The Involvement of RAGE and Its Ligands during Progression of ALS in SOD1 G93A Transgenic Mice.
Int J Mol Sci. 23 (4): 2184' -
Hu, X. et al. (2022) MiR-302d inhibits TGFB-induced EMT and promotes MET in primary human RPE cells.
PLoS One. 17 (11): e0278158. -
Zuniga, O. et al. (2022) Discovery of the inhibitor of DNA binding 1 as a novel marker for radioresistance in pancreatic cancer using genome-wide RNA-seq
Cancer Drug Resistance. 5: 926-38. -
Walter, M. et al. (2023) NUDT22 promotes cancer growth through pyrimidine salvage.
Oncogene. Mar 04 [Epub ahead of print]. -
Hirano, T. et al. (2023) An adverse outcome pathway-based approach to assess the neurotoxicity by combined exposure to current-use pesticides.
Toxicology. 500: 153687. -
Delfino, D. et al. (2024) Allergenicity of tropomyosin variants identified in the edible insect Hermetia illucens (black soldier fly)
Food Chemistry. 437: 137849. -
Garg, S. & , Miousse, I.R. (2024) Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line
Nutrients. 16 (7): 997.
- RRID
- AB_2721073
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