Browsing Modes for Business Users

Goat ANTI-RABBIT IgG StarBright™ Blue 700

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

Product Code Applications Pack Size List Price Your Price Qty
12004162
Datasheet Datasheet
SDS Safety Datasheet SDS
F WB 80 µl loader
List Price Your Price
loader
12004161
Datasheet Datasheet
SDS Safety Datasheet SDS
F WB 400 µl loader
List Price Your Price
loader

StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.

StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Goat Anti-Rabbit IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, mouse and rat.

Target Species
Rabbit
Product Form
Purified IgG conjugated to StarBright Blue 700 - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
Antiserum Preparation
Antiserum to Rabbit IgG was raised by repeated immunisation of Goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Blue 700 470 700
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/2,500 1/5,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing.
Instructions For Use
12004162, 12004161
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004158 F WB 400 µl loader
List Price Your Price
loader
Description Goat ANTI-MOUSE IgG StarBright™ Blue 700
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004159 F WB 80 µl loader
List Price Your Price
loader
Description Goat ANTI-MOUSE IgG StarBright™ Blue 700
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004163 WB 200 µl loader
List Price Your Price
loader
Description ANTI-ACTIN hFAB™ Rhodamine Antibody
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004164 WB 40 µl loader
List Price Your Price
loader
Description ANTI-ACTIN hFAB™ Rhodamine Antibody
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004165 WB 200 µl loader
List Price Your Price
loader
Description ANTI-TUBULIN hFAB™ Rhodamine Antibody
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004166 WB 40 µl loader
List Price Your Price
loader
Description ANTI-TUBULIN hFAB™ Rhodamine Antibody
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004167 WB 200 µl loader
List Price Your Price
loader
Description ANTI-GAPDH hFAB™ Rhodamine Antibody
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004168 WB 40 µl loader
List Price Your Price
loader
Description ANTI-GAPDH hFAB™ Rhodamine Antibody
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)

References for IgG antibody

  1. Li, T. et al. (2021) RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.
    Nat Commun. 12 (1): 1055.
  2. Vasileva, L.V. et al. (2021) Rosmarinic acid attenuates obesity and obesity-related inflammation in human adipocytes.
    Food Chem Toxicol. 149: 112002.
  3. Dunbar, K. et al. (2021) IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.
    Life Sci Alliance.4 (2): e202000804.
  4. Dunbar, K. et al. (2021) FAM83F regulates canonical Wnt signalling through an interaction with CK1&alpha.
    Life Sci Alliance. 4 (2): e202000805.
  5. Holoch, D. et al. (2021) A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals.
    Nat Genet. 53 (12): 1686-1697.
  6. Goad, D.W. et al. (2022) Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus.
    Mol Ther Oncolytics. 24: 59-76.
  7. Reisman, B.J. et al. (2022) Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase.
    Nat Chem Biol. 18 (4): 360-367.
  8. Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
    Life Sci Alliance. 5 (3): e202101185.
  9. View The Latest Product References
  10. Meissner, M.E. et al. (2022) Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.
    J Mol Biol. 434 (2): 167355.
  11. Nowicka, N. et al. (2022) The Involvement of RAGE and Its Ligands during Progression of ALS in SOD1 G93A Transgenic Mice.
    Int J Mol Sci. 23 (4): 2184
  12. Hu, X. et al. (2022) MiR-302d inhibits TGFB-induced EMT and promotes MET in primary human RPE cells.
    PLoS One. 17 (11): e0278158.
  13. Zuniga, O. et al. (2022) Discovery of the inhibitor of DNA binding 1 as a novel marker for radioresistance in pancreatic cancer using genome-wide RNA-seq
    Cancer Drug Resistance. 5: 926-38.
  14. Walter, M. et al. (2023) NUDT22 promotes cancer growth through pyrimidine salvage.
    Oncogene. 42 (16): 1282-1293.
  15. Hirano, T. et al. (2023) An adverse outcome pathway-based approach to assess the neurotoxicity by combined exposure to current-use pesticides.
    Toxicology. 500: 153687.
  16. Delfino, D. et al. (2024) Allergenicity of tropomyosin variants identified in the edible insect Hermetia illucens (black soldier fly)
    Food Chemistry. 437: 137849.
  17. Garg, S. & , Miousse, I.R. (2024) Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line
    Nutrients. 16 (7): 997.
  18. Anoud, M. et al. (2024) Comparative transcriptomics reveal a novel tardigrade-specific DNA-binding protein induced in response to ionizing radiation.
    Elife. 13: RP92621.
  19. Redondo, P.C. et al. (2024) Extended Synaptotagmins 1 and 2 Are Required for Store-Operated Calcium Entry, Cell Migration and Viability in Breast Cancer Cells.
    Cancers (Basel). 16 (14): 2518.
  20. Brar, K.K. et al. (2024) PERK-ATAD3A interaction provides a subcellular safe haven for protein synthesis during ER stress.
    Science. 385 (6712): eadp7114.
  21. Todorova, M.N. et al. (2024) Punica granatum L. leaf extract enhances stress tolerance and promotes healthy longevity through HLH-30/TFEB, DAF16/FOXO, and SKN1/NRF2 crosstalk in Caenorhabditis elegans.
    Phytomedicine. 134: 155971.
  22. DeMarco, A.G. et al. (2024) Inducible degradation-coupled phosphoproteomics identifies PP2A(Rts1) as a novel eisosome regulator.
    Front Cell Dev Biol. 12: 1451027.
  23. Smith-Davidson, P. et al. (2024) Prostaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.
    Laryngoscope. Nov 02 [Epub ahead of print].
  24. Zaric, V. et al. (2024) RNAi Knockdown of EHMT2 in Maternal Expression of Prader–Willi Syndrome Genes
    Genes. 15 (11): 1366.
  25. Khaerani, P.I. et al. (2024) Effect of UV-B stress on olive (Olea europaea L.) pollen tubes: A study of callose plug deposition and male germ unit integrity.
    Protoplasma. Nov 28 [Epub ahead of print].
  26. Hartmann, J. et al. (2024) SKA2 enhances stress-related glucocorticoid receptor signaling through FKBP4-FKBP5 interactions in neurons.
    Proc Natl Acad Sci U S A. 121 (52): e2417728121.
  27. Smith, S. et al. (2025) Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum.
    Hypertens Pregnancy. 44 (1): 2454597.
  28. Fuchs, H. et al. (2025) Selective toxicity of ascorbic acid and hydrogen peroxide on human tenon cells without harming scleral cells in vitro: A possible alternative to non-selective mitomycin C?
    PLoS One. 20 (4): e0320558.
  29. Fischer, A.S. et al. (2025) TGF-β Drives Myofibroblast Activation and Inflammatory Mediator Production in Vulvar Lichen Sclerosus
    AJOG Global Reports. : 100528.
  30. Leem, J. et al. (2025) A versatile cohesion manipulation system probes female reproductive age-related egg aneuploidy.
    Nat Aging. 5 (11): 2215-27.
  31. Machowicz, J. et al. (2025) Serotonin Signaling Pathway Modulation Affects Retinal Neuron Survival in Experimental Model of Retinal Ischemia.
    Life (Basel). 15 (11): 1726.
  32. Kunimatsu, R. et al. (2025) Houttuynia cordata Exhibits Anti-Inflammatory Activity Against Interleukin-1β-Induced Inflammation in Human Gingival Epithelial Cells: An In Vitro Study.
    Dent J (Basel). 13 (8): 360.
  33. Devaux, F. et al. (2025) Androgen receptor blockade and its effect on PSMA-localization in prostate cancer: Implications for radioligand therapy.
    Biomed Pharmacother. 193: 118849.
  34. Nieto-Felipe, J. et al. (2025) Notch1 regulates Orai1 and Orai3 expression in breast cancer cells.
    Sci Rep. Dec 18 [Epub ahead of print].
  35. Monaco, C.M. et al. (2025) A 5'-(R)-CH3-substituted 5-fluoro-2'-deoxyuridine monophosphate reduces off-target toxicities while maintaining efficacy in a colorectal cancer model.
    J Pharmacol Exp Ther. 393 (1): 103773.
  36. Wang, S.Z. et al. (2026) Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
    Cell Rep. 45 (3): 116965.
  37. Pejler, G. et al. (2026) Mast Cell Chymase and Human Lung Fibroblast Interaction: Mechanisms and Implications for Asthma.
    Inflammation. 49 (1): 88.
  38. Pavelich, L. et al. (2026) Integrative Proteomic and Bioenergetic Profiling Reveals Diet- and Strain-Specific Mitochondrial Dysfunction in Cohen Diabetic Rat Hearts.
    Antioxidants (Basel). 15 (2): 248.

RRID
AB_2721073
View more products with IgG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rabbit Products