IgG antibody
Goat ANTI-RABBIT IgG StarBright™ Blue 700
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Filter by Application:
WB Reset| StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores. StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot. Features and Benefits Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm) Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies Goat Anti-Rabbit IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, mouse and rat. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
- Antiserum Preparation
- Antiserum to Rabbit IgG was raised by repeated immunisation of Goats with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Blue 700 470 700 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Western Blotting | 1/2,500 | 1/5,000 |
- Western Blotting
- StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing. - Instructions For Use
-
12004162, 12004161
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
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Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
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View The Latest Product References
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- RRID
- AB_2721073
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