TRIAD3A antibody

Rabbit anti Mouse TRIAD3A (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TRIAD3A
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1000
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Human TRIAD3A antibody recognizes an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response.

Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to mouse TRIAD3A (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
Immunogen
A 15 amino acid peptide located near mouse TRIAD3A amino-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1000 detects a band of approximately 95kDa in mouse heart cell lysates.

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Further Reading

  1. Chuang, T.H. & Ulevitch, R.J. (2004) Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors.
    Nat Immunol. 5 (5): 495-502.
  2. Brint, E.K. et al. (2004) ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance.
    Nat Immunol. 5 (4): 373-9.

Synonyms
E3 ubiquitin-protein Ligase RNF216
RRID
AB_609826
UniProt
P58283
Entrez Gene
Rnf216
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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