- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide.
- Antiserum Preparation
- Antisera to mouse TNFα were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 6 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
positive regulation of chronic inflammatory response to antigenic stimulus
positive regulation of humoral immune response mediated by circulating immunoglobulin
integral to plasma membrane
tumor necrosis factor receptor binding
stored secretory granule
glucose metabolic process
transformed cell apoptosis
humoral immune response
defense response to Gram-positive bacterium
induction of apoptosis via death domain receptors
external side of plasma membrane
extracellular matrix organization
positive regulation of fever generation
positive regulation of interferon-gamma production
positive regulation of interleukin-6 production
tumor necrosis factor-mediated signaling pathway
negative regulation of growth of symbiont in host
positive regulation of I-kappaB kinase/NF-kappaB cascade
regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of translational initiation by iron
negative regulation of glucose import
positive regulation of JNK cascade
regulation of immunoglobulin secretion
positive regulation of hair follicle development
epithelial cell proliferation involved in salivary gland morphogenesis
regulation of branching involved in salivary gland morphogenesis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of TNF Alpha antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Rabbit anti Mouse TNFα (purified) may be used in an indirect ELISA with recombinant mouse TNFα (PMP24) as the standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for TNF Alpha antibody
Khaskhely, N.M. et al. (2002) Low-dose UVB contributes to host resistance against Leishmania amazonensis infection in mice through induction of gamma interferon and tumor necrosis factor alpha cytokines.
Clin Diagn Lab Immunol. 9: 677-86.
Schafers, M. et al. (2002) Anterograde transport of tumor necrosis factor-alpha in the intact and injured rat sciatic nerve.
J Neurosci. 22: 536-45.
Oakley, F. et al. (2005) Nuclear factor-kappaB1 (p50) limits the inflammatory and fibrogenic responses to chronic injury.
Am J Pathol. 166: 695-708.
Van Horssen, R. et al. (2006) TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility.
The Oncologist. 11: 397-408.
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