TNF Alpha antibody
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Rabbit anti Mouse TNF alpha antibody recognizes mouse Tumour Necrosis Factor alpha (TNF–alpha), a 17.3kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.
TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide. - Antiserum Preparation
- Antisera to mouse TNFα were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant mouse TNFα (PMP24)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 6 months from date of reconstitution
More Information
- UniProt
- P06804 Related reagents
- Entrez Gene
- Tnf Related reagents
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0001891 phagocytic cup
- GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0006006 glucose metabolic process
- GO:0006927 transformed cell apoptosis
- GO:0006959 humoral immune response
- GO:0007254 JNK cascade
- GO:0009887 organ morphogenesis
- GO:0050830 defense response to Gram-positive bacterium
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0009897 external side of plasma membrane
- GO:0030198 extracellular matrix organization
- GO:0030316 osteoclast differentiation
- GO:0031622 positive regulation of fever generation
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045121 membrane raft
- GO:0045670 regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0045994 positive regulation of translational initiation by iron
- GO:0046325 negative regulation of glucose import
- GO:0046330 positive regulation of JNK cascade
- GO:0051023 regulation of immunoglobulin secretion
- GO:0051798 positive regulation of hair follicle development
- GO:0055037 recycling endosome
- GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
- GO:0060693 regulation of branching involved in salivary gland morphogenesis
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 0.01 | 0.015ug/ml | |
| Immunohistology - Frozen | |||
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- ELISA
- Rabbit anti Mouse TNFα (purified) may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with recombinant mouse TNFα (PMP24) as the standard and Rabbit anti Mouse TNFα (biotin conjugate) (AAM19B) as the detection reagent.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for TNF Alpha antibody
-
Khaskhely, N.M. et al. (2002) Low-dose UVB contributes to host resistance against Leishmania amazonensis infection in mice through induction of gamma interferon and tumor necrosis factor alpha cytokines.
Clin Diagn Lab Immunol. 9: 677-86. -
Schafers, M. et al. (2002) Anterograde transport of tumor necrosis factor-alpha in the intact and injured rat sciatic nerve.
J Neurosci. 22: 536-45. -
Oakley, F. et al. (2005) Nuclear factor-kappaB1 (p50) limits the inflammatory and fibrogenic responses to chronic injury.
Am J Pathol. 166: 695-708.
Further Reading
-
Van Horssen, R. et al. (2006) TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility.
The Oncologist. 11: 397-408.
