Stem Cell Factor antibody
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Rabbit anti Mouse Stem Cell Factor
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Stem Cell Factor
Antibody Quality and Performance Guarantee
| Rabbit anti Mouse stem cell factor antibody recognizes mouse stem cell factor (SCF). SCF is a stromal cell derived cytokine that synergizes with other haemapoietic growth factors to stimulate the proliferation of myeloid and lymphoid progenitors in bone marrow culture. |
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse SCF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse SCF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
0.60 | 0.65ug/ml |
| Immunocytochemistry | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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References for Stem Cell Factor antibody
-
Chou, S. et al. (2013) Fetal Hepatic Progenitors Support Long-term Expansion of Hematopoietic Stem Cells.
Exp Hematol. pii: S0301-472X(13)00016-7.
- Synonyms
- SCF
- RRID
- AB_2131595
- UniProt
- P20826
- Entrez Gene
- Kitl
- GO Terms
- GO:0005886 plasma membrane
- GO:0001755 neural crest cell migration
- GO:0002763 positive regulation of myeloid leukocyte differentiation
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005173 stem cell factor receptor binding
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- View More GO Terms
- GO:0005856 cytoskeleton
- GO:0007281 germ cell development
- GO:0008083 growth factor activity
- GO:0033026 negative regulation of mast cell apoptosis
- GO:0035234 germ cell programmed cell death
- GO:0043406 positive regulation of MAP kinase activity
- GO:0045636 positive regulation of melanocyte differentiation
- GO:0046579 positive regulation of Ras protein signal transduction
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0070668 positive regulation of mast cell proliferation
AAM50
170324
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