Rabbit anti Mouse stem cell factor antibody recognizes mouse stem cell factor (SCF).
SCF is a stromal cell derived cytokine that synergizes with other haemapoietic growth factors to stimulate the proliferation of myeloid and lymphoid progenitors in bone marrow culture.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Antiserum Preparation
- Antisera to mouse SCF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant mouse SCF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
neural crest cell migration
positive regulation of myeloid leukocyte differentiation
integral to membrane
stem cell factor receptor binding
germ cell development
growth factor activity
negative regulation of mast cell apoptosis
germ cell programmed cell death
positive regulation of MAP kinase activity
positive regulation of melanocyte differentiation
positive regulation of Ras protein signal transduction
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Stem Cell Factor antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Stem Cell Factor antibody
Chou, S. et al. (2013) Fetal Hepatic Progenitors Support Long-term Expansion of Hematopoietic Stem Cells.
Exp Hematol. pii: S0301-472X(13)00016-7.