Stem Cell Factor antibody

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Rabbit anti Mouse Stem Cell Factor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM50 E FN IC WB 0.1 mg
Rabbit anti Mouse stem cell factor antibody recognizes mouse stem cell factor (SCF).

SCF is a stromal cell derived cytokine that synergizes with other haemapoietic growth factors to stimulate the proliferation of myeloid and lymphoid progenitors in bone marrow culture.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse SCF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant mouse SCF.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P20826 Related reagents
Entrez Gene
Kitl Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001755 neural crest cell migration
GO:0002763 positive regulation of myeloid leukocyte differentiation
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005173 stem cell factor receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0007281 germ cell development
GO:0008083 growth factor activity
GO:0033026 negative regulation of mast cell apoptosis
GO:0035234 germ cell programmed cell death
GO:0043406 positive regulation of MAP kinase activity
GO:0045636 positive regulation of melanocyte differentiation
GO:0046579 positive regulation of Ras protein signal transduction
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0070668 positive regulation of mast cell proliferation
Regulatory
For research purposes only

Applications of Stem Cell Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.60 0.65ug/ml
Immunocytochemistry
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Stem Cell Factor antibody

  1. Chou, S. et al. (2013) Fetal Hepatic Progenitors Support Long-term Expansion of Hematopoietic Stem Cells.
    Exp Hematol. pii: S0301-472X(13)00016-7.