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ST2 antibody

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Anti Mouse ST2 (N-Terminal) Antibody thumbnail image 1
Anti Mouse ST2 (N-Terminal) Antibody gallery image 1
Western blot analysis of mouse kidney lysate probed with Rabbit anti Mouse ST2 (AHP999) in the absence (A) or presence (B) of blocking peptide.

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Rabbit anti Mouse ST2 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ST2
Region
(N-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP999
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Mouse ST2 (N-Terminal) antibody recognizes an epitope within the N-Terminal region (NT) of mouse ST2, a member of the Toll-interleukin 1 receptor (TIR) superfamily, expressed by mast cells and TH2 helper cells, but not by TH1 effector cells.

The majority of TIR superfamily members, which includes the Interleukin-1 receptor (IL-1R1), Toll-like receptors (TLRs) and the cytosolic adaptor proteins MyD88, TIRAP, Trif and TRAM, initiate innate immunity through the ultimate activation of the transcription factor NF-kappaB and are inducers of the inflammatory response. In contrast ST2, which itself is unable to activate NF-kappaB, has been identified as a negative regulator of IL-1R1 and TLR4 signaling by sequestering MyD88 and TIRAP in line with its role as a crucial receptor in TH2-cell mediated responses (Brint et al. 2004 and Coyle et al. 1999).

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to mouse ST2 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
Immunogen
A 16 amino acid synthetic peptide located near mouse ST2 amino-terminus. This peptide is common to the three known isoforms of ST2.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP999 detecs a band of approximately 45kDa in mouse kidney cell lysates. (Predicted Mwt. 62 kDa).

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Further Reading

  1. Brint, E.K. et al. (2004) ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance.
    Nat Immunol. 5 (4): 373-9.
  2. Coyle, A.J. et al. (1999) Crucial role of the interleukin 1 receptor family member T1/ST2 in T helper cell type 2-mediated lung mucosal immune responses.
    J Exp Med. 190 (7): 895-902.

RRID
AB_2125319
UniProt
P14719
Entrez Gene
Il1rl1
GO Terms
GO:0002114 interleukin-33 receptor activity
GO:0002826 negative regulation of T-helper 1 type immune response
GO:0016021 integral to membrane
GO:0004908 interleukin-1 receptor activity
GO:0009897 external side of plasma membrane
GO:0032689 negative regulation of interferon-gamma production
GO:0032754 positive regulation of interleukin-5 production
GO:0043032 positive regulation of macrophage activation
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0050729 positive regulation of inflammatory response
GO:0090197 positive regulation of chemokine secretion

AHP999

152855

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