Rabbit anti Mouse ST2 (N-Terminal) antibody
recognizes an epitope within the N-Terminal region (NT) of mouse ST2, a member of the Toll-interleukin 1 receptor (TIR) superfamily, expressed by mast cells and TH2
helper cells, but not by TH1
The majority of TIR superfamily members, which includes the Interleukin-1 receptor (IL-1R1), Toll-like receptors (TLRs) and the cytosolic adaptor proteins MyD88, TIRAP, Trif and TRAM, initiate innate immunity through the ultimate activation of the transcription factor NF-kappaB and are inducers of the inflammatory response. In contrast ST2, which itself is unable to activate NF-kappaB, has been identified as a negative regulator of IL-1R1 and TLR4 signaling by sequestering MyD88 and TIRAP in line with its role as a crucial receptor in TH2
-cell mediated responses (Brint et al. 2004
and Coyle et al. 1999
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to mouse ST2 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- A 16 amino acid synthetic peptide located near mouse ST2 amino-terminus. This peptide is common to the three known isoforms of ST2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
interleukin-33 receptor activity
negative regulation of T-helper 1 type immune response
integral to membrane
interleukin-1 receptor activity
external side of plasma membrane
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB cascade
innate immune response
positive regulation of inflammatory response
positive regulation of chemokine secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ST2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP999 detecs a band of approximately 45kDa in mouse kidney cell lysates. (Predicted Mwt. 62 kDa).
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Brint, E.K. et al. (2004) ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance.
Nat Immunol. 5 (4): 373-9.
Coyle, A.J. et al. (1999) Crucial role of the interleukin 1 receptor family member T1/ST2 in T helper cell type 2-mediated lung mucosal immune responses.
J Exp Med. 190 (7): 895-902.
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