SIGIRR antibody

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Rabbit anti SIGIRR (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1784T
Datasheet
C WB
MSDS
50 µg loader

AHP1784
Datasheet
C WB
MSDS
0.1 mg loader

Rabbit anti SIGIRR (C-terminal) antibody recognizes the Single Ig IL-1-related receptor, also known as SIGIRR, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8 or TIR8. SIGIRR is a 409 amino acid ~ 45-90 kDa single pass type III transmembrane glycoprotein, Multiple species of SIGIRR seen in western blotting are achieved through differential glycosylation at four potential glycosylation sites in the SIGIRR ectodomain, mutation at these sites to eliminate glycosylation yields a single band of the predicted size (44 kDa) for unmodified SIGIRR (Zhao et al. 2015).

SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily, defined by an intracellular Toll-IL-1R (TIR) domain. SIGIRR acts as a negative regulator of the Toll-like and IL-1R receptor signalling pathways. It interacts with TLR4, TLR5, TLR9 and IL-1R through the extracellular Ig domain, or the intracellular TIR domain. The Ig domain interferes with the heterodimerization of IL-1R while the intracellular TIR domain inhibits both IL-1 and LPS signalling by attenuating the recruitment of receptor-proximal signalling components to TLR4. SIGIRR is expressed in kidney, colon, small intestine, lung, spleen and liver. It is not expressed in brain or muscle.

Rabbit anti SIGIRR antibody has been successfully used for the detection of SIGIRR in both murine and human samples by western blotting (Zhao et al. 2015)

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SIGIRR (CT) was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to 15 amino acids near the carboxy terminus of mouse SIGIRR.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9JLZ8
Q6IA17
Entrez Gene
SIGIRR
Sigirr
GO Terms
GO:0005515 protein binding
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0006953 acute-phase response
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0031665 negative regulation of lipopolysaccharide-mediated signaling pathway
GO:0043433 negative regulation of transcription factor activity
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of SIGIRR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 0.5 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1784 detects a band of approximately 43kDa in A549 cell lysate

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for SIGIRR antibody

  1. Zhao, J. et al. (2015) Human Colon Tumors Express a Dominant-Negative Form of SIGIRR That Promotes Inflammation and Colitis-Associated Colon Cancer in Mice.
    Gastroenterology. 149 (7): 1860-1871.e8.