SIGIRR antibody


SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily, defined by an intracellular Toll-IL-1R (TIR) domain. SIGIRR acts as a negative regulator of the Toll-like and IL-1R receptor signalling pathways. It interacts with TLR4, TLR5, TLR9 and IL-1R through the extracellular Ig domain, or the intracellular TIR domain. The Ig domain interferes with the heterodimerization of IL-1R while the intracellular TIR domain inhibits both IL-1 and LPS signalling by attenuating the recruitment of receptor-proximal signalling components to TLR4. SIGIRR is expressed in kidney, colon, small intestine, lung, spleen and liver. It is not expressed in brain or muscle.
Rabbit anti SIGIRR antibody has been successfully used for the detection of SIGIRR in both murine and human samples by western blotting (Zhao et al. 2015)
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SIGIRR (CT) was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to 15 amino acids near the carboxy terminus of mouse SIGIRR.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9JLZ8
- Q6IA17
- Entrez Gene
- SIGIRR
- Sigirr
- GO Terms
- GO:0005515 protein binding
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0006953 acute-phase response
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0031665 negative regulation of lipopolysaccharide-mediated signaling pathway
- GO:0043433 negative regulation of transcription factor activity
- GO:0045079 negative regulation of chemokine biosynthetic process
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of SIGIRR antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | 10ug/ml | ||
Western Blotting | 0.5 | 1ug/ml |
- Western Blotting
- AHP1784 detects a band of approximately 43kDa in A549 cell lysate
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
![]() |
|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for SIGIRR antibody
-
Zhao, J. et al. (2015) Human Colon Tumors Express a Dominant-Negative Form of SIGIRR That Promotes Inflammation and Colitis-Associated Colon Cancer in Mice.
Gastroenterology. 149 (7): 1860-1871.e8.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra