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SIGIRR antibody

Rabbit anti SIGIRR (C-Terminal)

Product Type
Polyclonal Antibody
Polyclonal IgG

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C WB 0.1 mg loader
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Rabbit anti SIGIRR (C-terminal) antibody recognizes the Single Ig IL-1-related receptor, also known as SIGIRR, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8 or TIR8. SIGIRR is a 409 amino acid ~ 45-90 kDa single pass type III transmembrane glycoprotein, Multiple species of SIGIRR seen in western blotting are achieved through differential glycosylation at four potential glycosylation sites in the SIGIRR ectodomain, mutation at these sites to eliminate glycosylation yields a single band of the predicted size (44 kDa) for unmodified SIGIRR (Zhao et al. 2015).

SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily, defined by an intracellular Toll-IL-1R (TIR) domain. SIGIRR acts as a negative regulator of the Toll-like and IL-1R receptor signalling pathways. It interacts with TLR4, TLR5, TLR9 and IL-1R through the extracellular Ig domain, or the intracellular TIR domain. The Ig domain interferes with the heterodimerization of IL-1R while the intracellular TIR domain inhibits both IL-1 and LPS signalling by attenuating the recruitment of receptor-proximal signalling components to TLR4. SIGIRR is expressed in kidney, colon, small intestine, lung, spleen and liver. It is not expressed in brain or muscle.

Rabbit anti SIGIRR antibody has been successfully used for the detection of SIGIRR in both murine and human samples by western blotting (Zhao et al. 2015)

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SIGIRR (CT) was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
A synthetic peptide corresponding to 15 amino acids near the carboxy terminus of mouse SIGIRR.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 0.5 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1784 detects a band of approximately 43kDa in A549 cell lysate

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References for SIGIRR antibody

  1. Zhao, J. et al. (2015) Human Colon Tumors Express a Dominant-Negative Form of SIGIRR That Promotes Inflammation and Colitis-Associated Colon Cancer in Mice.
    Gastroenterology. 149 (7): 1860-1871.e8.

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0006953 acute-phase response
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0031665 negative regulation of lipopolysaccharide-mediated signaling pathway
GO:0043433 negative regulation of transcription factor activity
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045087 innate immune response


146679 170116

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