PPAR Delta antibody

100% Secure

Rabbit anti PPAR Delta (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1272
Datasheet
E WB
MSDS
50 µg loader

Rabbit anti PPRAD antibody detects peroxisome proliferator-activated receptor, delta (PPARD), a member of the nuclear hormone receptor subfamily. Upon activation, PPARD forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. PPARD controls genes involved in lipid metabolism, and enhances fatty acid catabolism and energy uncoupling in adipose tissue and skeletal muscle.

PPARD has also been implicated in the proliferation and differentiation of preadipocytes, enterocytes, keratinocytes and the placenta.

PPARD agonists could be used therapeutically to target multiple components of the metabolic syndrome, including insulin resistance, hyperglycemia, dyslipidemia, and atherosclerosis.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit Expected from Sequence
Chimpanzee Expected from Sequence
Rat Expected from Sequence
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to PPAR delta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Syntetic peptide corresponding to amino acids 1-14 of mouse PPAR delta.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P35396 Related reagents
Entrez Gene
Ppard Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001890 placenta development
GO:0003707 steroid hormone receptor activity
GO:0005634 nucleus
GO:0006635 fatty acid beta-oxidation
GO:0007566 embryo implantation
GO:0050796 regulation of insulin secretion
GO:0008047 enzyme activator activity
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0008284 positive regulation of cell proliferation
GO:0008366 axon ensheathment
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0015908 fatty acid transport
GO:0016501 prostacyclin receptor activity
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030154 cell differentiation
GO:0031589 cell-substrate adhesion
GO:0042060 wound healing
GO:0042640 anagen
GO:0043565 sequence-specific DNA binding
GO:0043616 keratinocyte proliferation
GO:0045598 regulation of fat cell differentiation
GO:0046965 retinoid X receptor binding
GO:0046982 protein heterodimerization activity
GO:0050680 negative regulation of epithelial cell proliferation
GO:0051546 keratinocyte migration
GO:0060612 adipose tissue development
GO:0070539 linoleic acid binding
Regulatory
For research purposes only

Applications of PPAR Delta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/30000 1/70000
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1272 detects a band of approximately 55kDa in heart whole cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Luquet, S. et al. (2005) Roles of PPAR delta in lipid absorption and metabolism: a new target for the treatment of type 2 diabetes.
    Biochim Biophys Acta. 1740 (2): 313-7.
  2. Barish, G.D. et al. (2006) PPAR delta: a dagger in the heart of the metabolic syndrome.
    J Clin Invest. 116 (3): 590-7.
  3. Grimaldi, P.A. (2005) Regulatory role of peroxisome proliferator-activated receptor delta (PPAR delta) in muscle metabolism. A new target for metabolic syndrome treatment?
    Biochimie. 87 (1): 5-8.