PPAR Alpha antibody

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Rabbit anti PPAR Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1459 E P* WB 50 µg
Rabbit anti Mouse PPAR alpha antibody recognizes mouse Peroxisome proliferator-activated receptor alpha (PPAR ALPHA), a 52.2 kDa transcription factor belonging to the nuclear hormone receptor (NR1) family.

Peroxisome proliferator-activated receptors are vital in the regulation of cellular differentiation, development and metabolism. PPARA is also the target of hypolipidemic fibrates, a class of drugs used in cholesterol disorders.

PPARA alpha is expressed primarily in the liver, kidney, heart and skeletal muscle and is involved in the regulation of beta-oxidation of fatty acids.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Monkey
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse PPAR ALPHA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 1 to 18 of mouse PPAR alpha.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P23204 Related reagents
Entrez Gene
Ppara Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0005634 nucleus
GO:0006006 glucose metabolic process
GO:0008289 lipid binding
GO:0006631 fatty acid metabolic process
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0008544 epidermis development
GO:0016563 transcription activator activity
GO:0032099 negative regulation of appetite
GO:0042060 wound healing
GO:0043565 sequence-specific DNA binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046321 positive regulation of fatty acid oxidation
GO:0046982 protein heterodimerization activity
Regulatory
For research purposes only

Applications of PPAR Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Immunohistology - Paraffin 1 20ug/ml
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse liver

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000B 150 Tests C P