p38 Alpha MAPK antibody

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Rabbit anti p38 Alpha MAPK

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2410 WB 0.1 mg
Rabbit anti p38 alpha MAPK antibody recognizes mitogen-activated protein kinase p38 alpha (p38-alpha MAPK), also known as MAP kinase 14 (MAPK14) and stress activated protein kinase 2a (SAPK2a). p38 alpha is a member of the p38 MAPK serine/threonine kinase protein family, which also includes p38-beta (MAPK11), p38-gamma (MAPK12) and p38-delta (MAPK13) (Cargnello and Roux 2011).

p38 alpha MAPK becomes activated in response to a variety of stimuli, which include inflammatory cytokines and oxidative stress (Cargnello and Roux 2011). p38 alpha MAPK plays an essential role in mediating inflammation by regulating the expression of tumor necrosis factor alpha (TNF alpha) and interleukin 1 (IL-1) (Dhillon et al. 2007). p38 MAPK proteins have been implicated in cancer development and p38 alpha MAPK has been proposed to act as a tumor suppressor (Dhillon et al. 2007).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to p38 alpha MAPK was raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Peptide mapping to the carboxy terminus of mouse p38 alpha MAPK and sharing 100% homology with the human sequence
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P47811 Related reagents
Entrez Gene
Mapk14 Related reagents
Regulatory
For research purposes only

Applications of p38 Alpha MAPK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti p38 alpha MAPK recognizes a band of approximately 38 kDa when run under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*