Rabbit anti Nerve Growth Factor 2.5S antibody recognises murine Beta-nerve growth factor, also known as Nerve growth factor or NGF. Rabbit anti Nerve Growth Factor 2.5S antibody recognizes both the 2.5S and 7S forms of mouse nerve growth factor beta, a secreted peptide involved in the development and maintenance of the sympathetic and sensory nervous systems.
In a standard bioassay, this antibody neutralises the activity of 3.0 ng/ml NGF 2.5S when used at a concentration of around 0.5 μg/ml.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Antiserum Preparation
- Antiserum to NGF 2.5S was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Affinity purified mouse NGF 2.5S.
- Approx. Protein Concentrations
- IgG concentration1.0 mg/ml after reconstitution
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
receptor signaling protein activity
endoplasmic reticulum lumen
peripheral nervous system development
growth factor activity
sensory perception of pain
negative regulation of neuron apoptosis
positive regulation of axon extension
regulation of neurotransmitter secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Nerve Growth Factor 2.5S antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for Nerve Growth Factor 2.5S antibody
Ye, Y. et al. (2011) Nerve Growth Factor Links Oral Cancer Progression, Pain, and Cachexia
Mol Cancer Ther. 10: 1667-76.
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