MCP-1 antibody
Rabbit anti Mouse MCP-1 antibody (AAM43) used for the identification of MCP-1 expressing cells in murine glioma by indirect immunofluorescence on formailin fixed vibratome sections.
Image caption:
CCL2+ cells are GFAP+ astrocytes in human glioma.
(A) Confocal images show co-localization of CCL2-expressing cells (red) with GFAP (green) in glioma. Insert shows a detail of a single CCL2-expressing cell (red) co-localizing with GFAP (green) and counterstained with DAPI (blue). Scale bar: 30 μm. (B) Areas of infiltration of CD3+ T-cells (green) coincide with the areas of CCL2-expressing cells (red) in glioma. Confocal images show a tumorigenic area, infiltrated with CD3+ T-cells (green) in an area with numerous CCL2-expressing cells (red). The immunostaining was combined with a counterstaining with DAPI to detect the nucleus (blue). Scale bar: 50 μm. (C) Examples of characteristic CD3 infiltration in samples of glioma. CD3+ T-cells can be observed grouped in BVs (BV, limited by broken red line in 1 and 3) but also infiltrated in the parenchyma (2, 4, blue insert magnified in 5). (D) CCL2 expression correlates with the infiltration of T-cells in the tumor areas. The quantification in serial sections of the number of T-cells, either infiltrated or located in the BV lumen, revealed that the level of infiltration of CD3+ T-cells in the parenchyma (green) is positively correlated with the level of CCL2 expression (BV; BV lumen).
From: Citation: Carrillo-de Sauvage MA, Gómez A, Ros CM, Ros-Bernal F, Martín ED, Perez-Vallés A, et al. (2012)
CCL2-Expressing Astrocytes Mediate the Extravasation of T Lymphocytes in the Brain. Evidence from Patients with Glioma and Experimental Models In Vivo.
PLoS ONE 7(2): e30762. doi:10.1371/journal.pone.0030762.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse MCP-1 antibody (AAM43) used for the identification of MCP-1 expressing cells in murine glioma by immunohistochemistry on formailin fixed vibratome sections.
Image caption:
Increase in the number of CCL2+ cells in the mouse brain after LPS intrastriatal injection. (A) Representative picture of CCL2+ cells in the mouse striatum after LPS injection compared to a saline injection. (B) Quantification of CCL2+ cells in the injected striatum in a group of mice injected with saline compared with a group of mice injected with LPS. A dramatic increase of CCL2+ cells can be observed in the LPS injected mice. *p<0.05 Student t-test.
From: Citation: Carrillo-de Sauvage MA, Gómez A, Ros CM, Ros-Bernal F, Martín ED, Perez-Vallés A, et al. (2012)
CCL2-Expressing Astrocytes Mediate the Extravasation of T Lymphocytes in the Brain. Evidence from Patients with Glioma and Experimental Models In Vivo.
PLoS ONE 7(2): e30762. doi:10.1371/journal.pone.0030762.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse MCP-1 antibody (AAM43) used to evaluate MCP-1 expression in murine samples by western blotting.
Image caption:
Effect of ethanol on MCP-1 and CCR2 expression. a C57BL6 mice of postnatal day 4 (PD4) were exposed to ethanol (EtOH) as described in the “Methods” section. 8 h after EtOH exposure, mice were sacrificed and the brain was processed for immunoblotting (IB) analysis of MCP-1 and CCR2. The expression of MCP-1, MCPIP, and CCR2 in the brain was quantified and normalized to actin. Each data point was the mean ± SEM of six animals. b The expression of MCP-1 and CCR2 mRNA in the brain was determined by quantitative RT-PCR as described in the “Methods” section. Each data point was the mean ± SEM of three animals. ***p <0.001, statistically significant difference from control group. c SIM-A9 cells were exposed to EtOH (0.4%) for 3, 6, 12, or 12 h. The expression of MCP-1 and CCR2 was determined by IB and quantified by the normalization to the expression of actin. Each data point was the mean ± SEM of three independent experiments. *p <0.05, statistically significant difference from control group
From: Zhang K, Wang H, Xu M, Frank JA, Luo J.
Role of MCP-1 and CCR2 in ethanol-induced neuroinflammation and neurodegeneration in the developing brain.
J Neuroinflammation. 2018 Jul 5;15(1):197.
doi: 10.1186/s12974-018-1241-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Mouse MCP-1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MCP-1
Antibody Quality and Performance Guarantee
| Rabbit anti Mouse MCP-1 antibody recognizes mouse monocyte chemotactic protein-1 (MCP-1), also known as CCL2, Small-inducible cytokine A2 or Platelet-derived growth factor-inducible protein JE. Mouse MCP-1 is a 148 amino acid ~16 kDa secreted monocyte chemotactic factor The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts. MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages (Conti and DiGioacchino 2001). |
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse MCP-1 (PMP35)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
4.0 | 6.0ug/ml |
| Immunofluorescence | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- ELISA
- This product may be used in an indirect ELISA together with PMP35 as the standard.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for MCP-1 antibody
-
Tesch GH et al. (1999) Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis.
J Clin Invest. 103:73-80 -
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40. -
Daugherty, A. et al. (2010) Angiotensin II infusion promotes ascending aortic aneurysms: attenuation by CCR2 deficiency in apoE-/- mice.
Clin Sci (Lond). 118 (11): 681-9. -
Zhang, L. et al. (2012) Intra-amniotic administration of E coli lipopolysaccharides causes sustained inflammation of the fetal skin in sheep.
Reprod Sci. 19 (11): 1181-9. -
Carrillo-de Sauvage, M.A. et al. (2012) CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo.
PLoS One. 7: e30762 -
Ji, Z. et al. (2014) Thiamine Deficiency Promotes T Cell Infiltration in Experimental Autoimmune Encephalomyelitis: The Involvement of CCL2.
J Immunol. 193: 2157-67. -
Zhang, K. et al. (2018) Role of MCP-1 and CCR2 in ethanol-induced neuroinflammation and neurodegeneration in the developing brain.
J Neuroinflammation. 15 (1): 197. -
Ji, Z. et al. (2019) Obesity Promotes EAE Through IL-6 and CCL-2-Mediated T Cells Infiltration.
Front Immunol. 10: 1881.
View The Latest Product References
-
Ren, Z. et al. (2019) Minocycline attenuates ethanol-induced cell death and microglial activation in the developing spinal cord.
Alcohol. 79: 25-35. -
Rong, Y. et al. (2023) Engineered extracellular vesicles for delivery of siRNA promoting targeted repair of traumatic spinal cord injury.
Bioact Mater. 23: 328-42.
- Synonyms
- CCL2
- RRID
- AB_2071666
- UniProt
- P10148
- Entrez Gene
- Ccl2
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0010574 regulation of vascular endothelial growth factor production
- GO:0010759 positive regulation of macrophage chemotaxis
- GO:0016525 negative regulation of angiogenesis
- GO:0090265 positive regulation of immune complex clearance by monocytes and macrophages
AAM43
157569
158762
160928
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