Rabbit anti Mouse MCP-1 antibody
recognizes mouse monocyte chemotactic protein-1 (MCP-1), also known as CCL2, Small-inducible cytokine A2 or Platelet-derived growth factor-inducible protein JE. Mouse MCP-1 is a 148 amino acid ~16 kDa secreted monocyte chemotactic factor
The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.
MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages (Conti and DiGioacchino 2001
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Antiserum Preparation
- Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant mouse MCP-1 (PMP35)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
negative regulation of angiogenesis
positive regulation of immune complex clearance by monocytes and macrophages
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MCP-1 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- This product may be used in an indirect ELISA together with PMP35 as the standard.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for MCP-1 antibody
Tesch GH et al. (1999) Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis.
J Clin Invest. 103:73-80
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40.
Carrillo-de Sauvage, M.A. et al. (2012) CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo.
PLoS One. 7: e30762
Ji, Z. et al. (2014) Thiamine Deficiency Promotes T Cell Infiltration in Experimental Autoimmune Encephalomyelitis: The Involvement of CCL2.
J Immunol. 193: 2157-67.
Zhang, L. et al. (2012) Intra-amniotic administration of E coli lipopolysaccharides causes sustained inflammation of the fetal skin in sheep.
Reprod Sci. 19 (11): 1181-9.
Daugherty, A. et al. (2010) Angiotensin II infusion promotes ascending aortic aneurysms: attenuation by CCR2 deficiency in apoE-/- mice.
Clin Sci (Lond). 118 (11): 681-9.
Ren, Z. et al. (2019) Minocycline attenuates ethanol-induced cell death and microglial activation in the developing spinal cord.
Alcohol. 79: 25-35.