MCP-1 antibody

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Rabbit anti Mouse MCP-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM43 E FN IF WB 0.1 mg
Rabbit anti Mouse MCP-1 antibody recognizes mouse monocyte chemotactic protein-1 (MCP-1), also known as CCL2, Small-inducible cytokine A2 or Platelet-derived growth factor-inducible protein JE. Mouse MCP-1 is a 148 amino acid ~16 kDa secreted monocyte chemotactic factor

The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.

MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant mouse MCP-1 (PMP35)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P10148 Related reagents
Entrez Gene
Ccl2 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010759 positive regulation of macrophage chemotaxis
GO:0016525 negative regulation of angiogenesis
GO:0090265 positive regulation of immune complex clearance by monocytes and macrophages
Regulatory
For research purposes only

Applications of MCP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 4.0 6.0ug/ml
Immunofluorescence
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA together with PMP35 as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for MCP-1 antibody

  1. Tesch GH et al. (1999) Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis.
    J Clin Invest. 103:73-80
  2. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
    Curr Alzheimer Res. 6: 531-40.
  3. Carrillo-de Sauvage, M.A. et al. (2012) CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo.
    PLoS One. 7: e30762
  4. Ji, Z. et al. (2014) Thiamine Deficiency Promotes T Cell Infiltration in Experimental Autoimmune Encephalomyelitis: The Involvement of CCL2.
    J Immunol. 193: 2157-67.
  5. Zhang, L. et al. (2012) Intra-amniotic administration of E coli lipopolysaccharides causes sustained inflammation of the fetal skin in sheep.
    Reprod Sci. 19 (11): 1181-9.
  6. Daugherty, A. et al. (2010) Angiotensin II infusion promotes ascending aortic aneurysms: attenuation by CCR2 deficiency in apoE-/- mice.
    Clin Sci (Lond). 118 (11): 681-9.