IL-9 antibody

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Rabbit anti Mouse Interleukin-9:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2810Z
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Mouse interleukin-9 antibody, recognizes interleukin-9 (IL-9), also known as cytokine P40 and T cell growth factor P40.

IL-9 is a member of the common gamma chain receptor cytokine family that is expressed by multiple cell types including T cell subsets, neutrophils, eosinophils, natural killer T cells and mast cells (Noelle & Nowak 2010, Chakraborty et al. 2019).

IL-9 is a ligand of IL-9 receptor alpha (IL-9RA) and upon binding of IL-9 to its receptor the JAK, PI3K, STAT and MAPK signaling pathways can be activated (Chakraborty et al. 2019). This can then result in the stimulation of expression of genes that play roles in transplant tolerance, allergies, autoimmunity and parasitic infection (Noelle & Nowak 2010).

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant mouse interleukin-9 and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant mouse interleukin-9
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P15247
Entrez Gene
Il9
GO Terms
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0045407 positive regulation of interleukin-5 biosynthetic process
Regulatory
For research purposes only

Applications of IL-9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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