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IgM antibody

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Anti Mouse IgM (Human Adsorbed) Antibody thumbnail image 1
Anti Mouse IgM (Human Adsorbed) Antibody gallery image 1
Published customer image:
APC conjugated Goat anti Mouse IgM antibody (102011L) used to detect bound anti CD$ IgM antibody by flow cytometery
Image caption:
Average percentage of peripheral blood mononuclear cells (PBMC) from s6823 (a) and sM2 (b) that stained positive for CD4 and interferon gamma (IFN-γ), and CD8 and IFN-γ expression markers after stimulation with Erum2510 peptide pools. Peripheral blood mononuclear cells were stimulated with 10 µg/mL protein and incubated for 72 h, after which the percentage of cells was determined by flow cytometry. Unstimulated PBMCs were included as negative control.

From: Ngoepe, T., Pretorius, A., Steyn, H., & van Kleef, M. (2023).
Th1 and Th2 epitopes of Cowdria polymorphic gene 1 of Ehrlichia ruminantium. Onderstepoort Journal of Veterinary Research, 90(1), 15 pages. doi: 10.4102/ojvr.v90i1.2070.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Mouse IgM:APC (Human Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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Product Code Applications Pack Size List Price Your Price Qty
102011S
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF 0.25 mg loader
List Price Your Price
loader
102011L
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF 0.5 mg loader
List Price Your Price
loader

Goat anti Mouse IgM antibody recognizes the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity is observed with human immunoglobulins.

This Goat anti Mouse IgM polyclonal antibody has been cross absorbed against Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.

Target Species
Mouse
Product Form
Purified Ig conjugated to Allophycocyanin (APC) - liquid
Preparation
Purified Ig prepared by affinity chromatography on IgM covalently linked to agarose
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Immunogen
Mouse IgM paraproteins.
Approx. Protein Concentrations
102011S: IgG concentration 0.5 mg/ml
102011L: Ig concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Pack Size: 0.25 mg
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry Neat 1/5
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

How to Use the Spectraviewer

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  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgM antibody

  1. Ngoepe, T.A. et al. (2023) Th1 and Th2 epitopes of Cowdria polymorphic gene 1 of Ehrlichia ruminantium.
    Onderstepoort J Vet Res. 90 (1): e1-e15.

RRID
AB_616699
UniProt
P01873
P01872
Entrez Gene
Ighm
Ighm
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane

102011S

167739 241114

102011L

158296 158620

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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