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IgG1 antibody

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Anti Mouse IgG1 Antibody thumbnail image 1
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Published customer image:
Biotinylated Goat anti Mouse IgG1 antibody (107008) used to detect bound Mouse anti Human synaptophysin antibody, clone SY38 (8479-0004) to identify synaptophysin in tongues of developinng fetus's by immunohistochemistry.
Image caption:
Tongue epithelium development:
3 (B) Age 20 weeks: Faint expression of Synaptophysin recorded (10x). 4 (C) Age 22 weeks: Faint expression of Synaptophysin is seen in the papilla (40x).

From:Sen, S., Mishra, S. & Kaul, J. M. (2014).
Study of Maturation of Synapses in the Human Tongue Epithelium Using Synaptophysin as Marker: An Immunohistochemical Approach.
Journal of Pioneering Medical Sciences, 4(3), 105-111.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Mouse IgG1:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG1

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Product Code Applications Pack Size List Price Your Price Qty
107008
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Goat anti Mouse IgG1 antibody recognizes mouse immunoglobulin isotype G1.

Goat anti Mouse IgG1 antibody has been adsorbed against mouse IgM, IgG2a, IgG2b, IgG3, IgA and human sera to minimise cross-reactivity.

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to mouse IgG1 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Mouse IgG1 covalently linked to agerose.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse IgG1 paraproteins.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG1 antibody

  1. Jackson, S.J. et al. (2004) Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture.
    Eur J Neurosci. 20: 2267-75.
  2. Kushwaha, V. & Kaur, S. (2021) Cross-protective efficacy of immuno-stimulatory recombinant Brugia malayi. protein HSP60 against the Leishmania donovani. in BALB/c mice.
    Biologicals. 72: 18-26.
  3. Sen, S., et al. (2014) Study of Maturation of Synapses in the Human Tongue Epithelium Using Synaptophysin as Marker: An Immunohistochemical Approach.
    Journal of Pioneering Medical Sciences, 4(3), 105-111.

RRID
AB_609714
UniProt
P01868
P01869
Entrez Gene
Ighg1
Ighg1
GO Terms
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0003823 antigen binding
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0005886 plasma membrane
GO:0016021 integral to membrane

107008

010615 163595

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