IgG1 antibody
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Goat anti Mouse IgG1 antibody recognizes mouse immunoglobulin isotype G1.
Goat anti Mouse IgG1 antibody has been adsorbed against mouse IgM, IgG2a, IgG2b, IgG3, IgA and human sera to minimise cross-reactivity.
Goat anti Mouse IgG1 antibody has been adsorbed against mouse IgM, IgG2a, IgG2b, IgG3, IgA and human sera to minimise cross-reactivity.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Texas Red® - liquid
- Antiserum Preparation
- Antisera to mouse IgG1 were raised by repeated immunisation of goats with highly purified antigen. Purified Ig was prepared from whole serum by affinity chromatography on Mouse IgG1 covalently linked to agarose.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse IgG1 paraproteins.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01868
- P01869
- Entrez Gene
- Ighg1
- Ighg1
- GO Terms
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0003823 antigen binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0050766 positive regulation of phagocytosis
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- Acknowledgements
- Texas Red® is a registered trademark of Molecular Probes, Inc.
- Regulatory
- For research purposes only
Applications of IgG1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/10,000 | |
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG1 antibody
-
Jackson, S.J. et al. (2004) Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture.
Eur J Neurosci. 20: 2267-75. -
Kushwaha, V. & Kaur, S. (2021) Cross-protective efficacy of immuno-stimulatory recombinant Brugia malayi. protein HSP60 against the Leishmania donovani. in BALB/c mice.
Biologicals. 72: 18-26.
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