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IgG1 antibody

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Goat anti Mouse IgG1:Texas Red®

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG1

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Goat anti Mouse IgG1 antibody recognizes mouse immunoglobulin isotype G1.

Goat anti Mouse IgG1 antibody has been adsorbed against mouse IgM, IgG2a, IgG2b, IgG3, IgA and human sera to minimise cross-reactivity.

Target Species
Mouse
Product Form
Purified IgG conjugated to Texas Red - liquid
Antiserum Preparation
Antisera to mouse IgG1 were raised by repeated immunisation of goats with highly purified antigen. Purified Ig was prepared from whole serum by affinity chromatography on Mouse IgG1 covalently linked to agarose.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3).
Immunogen
Mouse IgG1 paraproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Texas Red 596 615
Regulatory
For research purposes only.
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Acknowledgements
Texas Red is a trademark of Molecular Probes, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgG1 antibody

  1. Jackson, S.J. et al. (2004) Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture.
    Eur J Neurosci. 20: 2267-75.
  2. Kushwaha, V. & Kaur, S. (2021) Cross-protective efficacy of immuno-stimulatory recombinant Brugia malayi. protein HSP60 against the Leishmania donovani. in BALB/c mice.
    Biologicals. 72: 18-26.

RRID
AB_609718
UniProt
P01868
P01869
Entrez Gene
Ighg1
Ighg1
GO Terms
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0003823 antigen binding
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0005886 plasma membrane
GO:0016021 integral to membrane
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products