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IgG IgA IgM antibody

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Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG IgA IgM

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Product Code Applications Pack Size List Price Your Price Qty
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Goat anti Mouse IgG/A/M (Human Adsorbed) antibody recognizes all subclasses of murine immunoglobulin and has been adsorbed against human, bovine and hamster immunoglobulins.

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to mouse Ig were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Mouse IgG purified from normal mouse serum, mouse IgA and IgM purified from ascitic fluid.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/3000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG IgA IgM antibody

  1. Bergmeier, L.A. et al. (2005) Mucosal alloimmunization elicits T-cell proliferation, CC chemokines, CCR5 antibodies and inhibition of simian immunodeficiency virus infectivity.
    J Gen Virol. 86: 2231-8.
  2. Peters, B. et al. (2004) Effect of heterosexual intercourse on mucosal alloimmunisation and resistance to HIV-1 infection.
    Lancet. 363: 518-24.
  3. Bartlomiejczyk, M.A. et al. (2014) Interaction of lectin pathway of complement-activating pattern recognition molecules with mycobacteria.
    Clin Exp Immunol. 178 (2): 310-9.
  4. Kubelkova, K. et al. (2021) Early infection-induced natural antibody response.
    Sci Rep. 11 (1): 1541.
  5. Grandoni, F. et al. (2017) Characterization of leukocyte subsets in buffalo (Bubalus bubalis.) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules.
    Dev Comp Immunol. 74: 101-109.

Flow Cytometry

RRID
AB_321854
UniProt
P01867
P01865
P01864
P01863
P01869
P01868
P03987
P01872
P01873
P01878
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
Ighm
Ighm
Igh-VJ558
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway

STAR87P

151605 162262 1806

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