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IgG antibody

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Goat anti Mouse IgG:HRP (Rat Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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C E P 0.5 mg loader
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Horseradish peroxidase conjugated Goat anti Mouse IgG antibody recognizes mouse IgG. Cross-reactivity with rat immunoglobulins has been reduced by solid phase adsorption.

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified mouse IgG.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunohistology - Frozen 1/50
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG antibody

  1. Yang, L.P. et al. (2009) Baicalein reduces inflammatory process in a rodent model of diabetic retinopathy.
    Invest Ophthalmol Vis Sci. 50: 2319-27.
  2. Bhrany, A.D. et al. (2008) Crosslinking of an oesophagus acellular matrix tissue scaffold.
    J Tissue Eng Regen Med. 2: 365-72.
  3. Sourial-Bassillious, N. et al. (2006) Effect of TNF-alpha on CD3-zeta and MHC-I in postnatal rat hippocampus.
    Pediatr Res. 60: 377-81.
  4. Madden, J. et al. (2009) Altered monocyte CD44 expression in peripheral arterial disease is corrected by fish oil supplementation.
    Nutr Metab Cardiovasc Dis. 19: 247-52.
  5. Liu Z et al. (2016) Partial protective immunity against toxoplasmosis in mice elicited by recombinant Toxoplasma gondii malate dehydrogenase.
    Vaccine. 34 (7): 989-94.
  6. Sessevmez, M. et al. (2023) Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
    J Drug Deliv Sci Technol. 86: 104704.

RRID
AB_321915
UniProt
P01869
P03987
P01864
P01868
P01865
P01867
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0006911 phagocytosis, engulfment
GO:0006910 phagocytosis, recognition
GO:0001812 positive regulation of type I hypersensitivity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0002455 humoral immune response mediated by circulating immunoglobulin

STAR77

158870 162261 1801

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